| 高效液相色谱法测定抗骨增生丸中淫羊藿苷的含量 |
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| 引用本文： | 蒋桂平.高效液相色谱法测定抗骨增生丸中淫羊藿苷的含量[J].中南药学,2008,6(3):322-323. |
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| 作者姓名： | 蒋桂平 |
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| 作者单位： | 湖南省涟钢医院,湖南,娄底,417009 |
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| 摘 要： | 目的建立抗骨增生丸中淫羊藿苷含量的高效液相色谱法测定方法。方法样品经处理后,由Diamonsil C18（4.6mm×200mm,5μm）色谱柱分离,流动相：乙腈-水（26∶74）,流速：1.0mL.min^-1,检测波长：270nm。结果方法分离良好,样品中杂质不干扰淫羊藿苷的分离测定,淫羊藿苷浓度在0.0404-0.504μg内与峰面积线性关系好,r=0.9999,平均回收率为98.4%,RSD为0.7%（n=6）。结论本法测定简便,结果准确,重复性和分离度好,可用于抗骨增生丸的质量控制。
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| 关 键 词： | 高效液相色谱法 淫羊藿苷 抗骨增生丸 含量测定 |
| 文章编号： | 1672-2981(2008)04-0322-02 |
| 修稿时间： | 2008年3月6日 |
Determination of icariin in kangguzengsheng pills by HPLC |
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| JIANG Gui-ping.Determination of icariin in kangguzengsheng pills by HPLC[J].Central South Pharmacy,2008,6(3):322-323. |
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| Authors: | JIANG Gui-ping |
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| Institution: | JIANG Gui-ping(Liangang Hospital of Hunan Province,Loudi Hunan 417009) |
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| Abstract: | Objective To develop an HPLC method for the determination of icariin in Kangguzengsheng pills.Methods A C18 column（4.6 mm×200 mm,5 μm）,and a mobile phase composed of acetonitrile-water（26∶74）were adopted.The flow rate was 1.0 mL·min^-1 and wave length was 270 nm.Results The linear range was 0.040 4～0.504 μg,r=0.999 98.The recovery of paeoniflorin was 98.4% with RSD=0.7%（n=6）.Conclusion The determination method is simple and accurate,and can be used for the quality control of Kangguzengsheng pills. |
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| Keywords: | HPLC icariin kangguzengsheng pills determination |
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