PPAR-γ在COPD所致的肺动脉高压患者中的表达变化

目的探讨COPD所致肺动脉高压患者PPAR-γ表达水平与肺动脉压力、胰岛素抵抗的关系。方法63例COPD患者中合并肺动脉高压（PAH组）30例，无肺动脉高压COPD组33例，20例体检者为对照组。采用实时荧光定量RT—PCR测定外周血单个核细胞PPAR-γmRNA表达水平，放免法检测空腹胰岛素（FIN），葡萄糖氧化酶法测定空腹血糖（FPG）。结果COPD组及PAH组PPAR-γ表达水平下降，FPG、FIN及IRI升高；PAH组肺动脉压力增高，PPAR-γ表达下降及IRI升高更加明显；PPAR-γ表达水平与肺动脉压力、FPG、FIN及IRI均呈负相关（r=-0．683，-0．476，-0．553，-0．589，P〈0．05），肺动脉压力与IRI呈正相关（r=0．505，P〈0．05）。结论PPAR-γ表达下调可能与空腹血糖异常、胰岛素抵抗、肺动脉高压形成有关。

The expression of PPAR-gamma in patients of pulmonary hypertension by chronic obstructive pulmonary disease

Objective To investigate the relationship among peroxisome proliferators - activated receptor gamma (PPAR-γ), pul-monary arterial systolic pressure(PASP) ,and insulin resistance in Chronic Obstructive Pulmonary Disease (COPD) patients. Methods A-mong 63 COPD patients, 30 patients with level of PASP above 40mmHg were enrolled in PAH group and other 33 patients were enrolled in COPD group. Twenty healthy medical examination subjects were enrolled in control group. The expression of PPAR-γ, mRNA was detected by real time fluorescent quantitative RT- PCR. Radioimmunoassay was used to measure the level of fasting plasma insulin (FIN). Fasting plas-ma glucose (FPG) was detected by glucose oxidase method. Results The expression of PPAR-γ mRNA was significantly decreased in PAH and COPD group, while FPG, FIN and IRI increased significantly. PAH group had more increased PASP, decreased expression of PPAR-γ and higher IRI than COPD group. Expression of PPAR-γ was negatively related to PASP and IRI. Conclusions The significantly down reg-ulated expression of PPAR-γ maybe explain the higher FPG and PASP.