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| BrdU标记胎兔骨髓间充质干细胞及对细胞生物学行为影响的体外研究 | |
| 引用本文： | 李德绘,黎洪棉,梁自乾,柳大烈. BrdU标记胎兔骨髓间充质干细胞及对细胞生物学行为影响的体外研究[J]. 广西医科大学学报, 2011, 28(4): 506-511 |
| 作者姓名： | 李德绘 黎洪棉 梁自乾 柳大烈 |
| 作者单位： | 1. 广西医科大学第一附属医院烧伤整形外科南宁 530021 2. 中山大学附属中山医院博士后科研工作站,整形美容外科 中山,528403 3. 南方医科大学附属珠江医院整形外科 广州510282 |
| 基金项目： | 广西自然科学基金资助项目(No.桂科自0991112) |
| 摘 要： | 目的:通过用5-溴脱氧尿苷(5- bromodeoxyuridine,BrdU)标记胎兔骨髓间充质干细胞(bone marrow rnesenchymal stem cells,BMSCs),探讨BrdU作为干细胞标记示踪方法的可行性.方法:取胎兔骨髓组织密度梯度离心法分离培养BMSCs.取P3代细胞以终浓度分别为5,... |
| 关 键 词： | 骨髓间充质干细胞 组织工程 尿嘧啶核苷酸类 细胞标记 细胞毒性 |
IN VIRTO BROMODEOXYURIDINE LABELING OF BONE MARROW MESENCHYMAL STEM CELLS FROM BREPHO-RABBIT |
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| Li Dehui,Li Hongmian,Liang Ziqian,Liu Dalie. IN VIRTO BROMODEOXYURIDINE LABELING OF BONE MARROW MESENCHYMAL STEM CELLS FROM BREPHO-RABBIT[J]. Journal of Guangxi Medical University, 2011, 28(4): 506-511 | |
| Authors: | Li Dehui Li Hongmian Liang Ziqian Liu Dalie |
| Affiliation: | Li Dehui,Li Hongmian,Liang Ziqian,Liu Dalie.(Department of Burn and Plastic Surgery,the First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China) |
| Abstract: | Objective:We explored the optimal dosage,timing and cytotoxicity for bromodeoxyurident(BrdU) labeling of brepho-rabbit bone marrow mesenchymal stem cells(BMSCs) in vitro,in order to confirm its feasibility for stem cells labeling and tracer means.Methods:Brepho-rabbit bone marrow was isolated and cultured the BMSCs using the density gradient centrifugation method in vitro.The three passages of BMSCs were incubated with BrdU at different concentrations(5,10,15 and 20 mg/L) for incubating time(12,24,48 and 72... |
| Keywords: | bone marrow mesenchymal stem cells(BMSCs) tissue engineering uracil mucleotides stem cell label cytotoxicity |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |