| 尿激酶对大鼠胸膜成纤维细胞增殖与细胞凋亡的影响 |
| |
| 引用本文： | 邵景韫,刘安,戚好文,董玉. 尿激酶对大鼠胸膜成纤维细胞增殖与细胞凋亡的影响[J]. 医学争鸣, 2006, 27(23): 2146-2149 |
| |
| 作者姓名： | 邵景韫 刘安 戚好文 董玉 |
| |
| 作者单位： | 西安市中心医院呼吸内科,陕西,西安,710003;第四军医大学西京医院呼吸内科,陕西,西安,710033 |
| |
| 基金项目： | 陕西省西安市科技攻关项目 |
| |
| 摘 要： | 目的:研究尿激酶(UK)对大鼠胸膜成纤维细胞(FB)增殖与细胞凋亡的影响. 方法:将大鼠胸膜FB原代培养,4～12代用于实验,FB与不同浓度UK共培养24～96 h;用光学显微镜观察其形态学改变;荧光染色查找凋亡小体;流式细胞技术观察有无凋亡细胞出现. 结果:UK以时间和浓度依赖的方式抑制细胞生长,但未发现凋亡小体,流式细胞技术没有检测到凋亡细胞. 结论:UK能够抑制FB增殖,其机制与细胞凋亡无关.
|
| 关 键 词： | 尿激酶 胸膜 成纤维细胞 细胞凋亡 |
| 文章编号： | 1000-2790(2006)23-2146-04 |
| 收稿时间： | 2006-04-11 |
| 修稿时间： | 2006-06-30 |
Effect of urokinase on cell proliferation and apoptosis of rat pleural fibroblasts |
| |
| SHAO Jing-Yun,LIU An,QI Hao-Wen,DONG Yu. Effect of urokinase on cell proliferation and apoptosis of rat pleural fibroblasts[J]. Negative, 2006, 27(23): 2146-2149 |
| |
| Authors: | SHAO Jing-Yun LIU An QI Hao-Wen DONG Yu |
| |
| Abstract: | AIM: To study the effect of urokinase on the cell proliferation and apoptosis of rat pleural fibroblasts. METHODS: Rat pleural fibroblasts were primarily cultured to 4-12 passages, and then the medium was added with urokinase of different concentrations. After another 24-96 h culture, light microscope was used to observe morphological change. Apoptosis bodies were examined by fluorescent staining. Flow cytometry was used to detect apoptotic cells. RESULTS: Urokinase decreased the number of viable cells in time and concentration dependent manners. No apoptosis body or apoptotic cells were found by flow cytometry. CONCLUSION: Antiproliferative effect of urokinase on rat pleural fibroblasts is confirmed, but not related to cell apoptosis. |
| |
| Keywords: | urokinase pleura fibroblasts apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
