| 弓形虫GRA7基因在大肠埃希菌中的表达 |
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| 引用本文： | 尹志奎,杨锦楠,许兵红,刘世国.弓形虫GRA7基因在大肠埃希菌中的表达[J].新乡医学院学报,2005,22(2):99-101. |
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| 作者姓名： | 尹志奎 杨锦楠 许兵红 刘世国 |
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| 作者单位： | 1. 新乡医学院药理学教研室,河南,新乡,453003
2. 新乡医学院寄生虫教研室,河南,新乡,453003 |
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| 摘 要： | 目的 在大肠埃希菌中表达致密颗粒蛋白(GRA7)。方法 将弓形虫GRA7基因克隆入原核表达载体p GEX- 4 T- 1,构建重组质粒p GEX- 4 T- 1/GRA7并转化大肠埃希菌BL2 1。IPTG体外诱导重组质粒菌的表达,对表达的GRA7融合蛋白进行SDS- PAGE和Western- blotting分析。结果 成功构建了p GEX- 4 T- 1/GRA7重组质粒,该重组质粒在大肠埃希菌中表达了目的蛋白,该蛋白能与抗GST抗体结合。结论 GRA7基因在大肠埃希菌中以GST融合蛋白的形式得到表达。
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| 关 键 词： | 弓形虫 致密颗粒蛋白7 蛋白质表达 |
| 文章编号： | 1004-7239(2005)02-0099-03 |
Expression of GRA7 gene of Toxoplasma gondii in Escherichia coli |
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| YIN Zhi-kui,YANG Jin-nan,XU Bing-hong,et al.Expression of GRA7 gene of Toxoplasma gondii in Escherichia coli[J].Journal of Xinxiang Medical College,2005,22(2):99-101. |
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| Authors: | YIN Zhi-kui YANG Jin-nan XU Bing-hong |
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| Institution: | YIN Zhi-kui1,YANG Jin-nan1,XU Bing-hong2,et al |
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| Abstract: | Objective To expressed GRA7 in Escherichia coli (E.coli).Methods GRA7 gene was cloned into the vector pGEX-4T-1.The recombinant plasmid pGEX-4T-1/ GRA7 was transformed into BL21 and induced by IPTG to express the fusion protein.This protein was identified by SDS-PAGE and Western-blotting.Results The recombinant plasmid pGEX-4T-1/GRA7 was constructed successfully and expressed the target protein in E.coli.This protein could react with anti-GST antibody.Conclusion The GRA7 gene may be expressed as a GST fusion protein in E.coli. |
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| Keywords: | Toxoplasma gondii dense granule protein7 protein expression |
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