Impact of APRF+ in Combination with Autogenous Fibroblasts on Release Growth Factors,Collagen, and Proliferation and Migration of Gingival Fibroblasts: An In Vitro Study |
| |
| Authors: | Barbara Sterczał a,Agnieszka Chwił kowska,Urszula Szwedowicz,Magdalena Kobielarz,Bartł omiej Chwił kowski,Marzena Dominiak |
| |
| Affiliation: | 1.Dental Surgery Department, Wroclaw Medical University, 50-425 Wroclaw, Poland;2.Department of Molecular and Cell Biology, Wroclaw Medical University, 50-556 Wroclaw, Poland;3.Department of Mechanics, Materials and Biomedical Engineering, Wroclaw University of Science and Technology, 50-371 Wroclaw, Poland;4.Department of Applied Mathematics, Faculty of Pure and Applied Mathematics, Wroclaw University of Science and Technology, 50-370 Wroclaw, Poland; |
| |
| Abstract: | The present study aimed to compare the action of advanced platelet-rich fibrin (A-PRF+) alone with the action of A-PRF+ combined with autologous gingival fibroblasts. The components released from A-PRF+ conditioned with autogenous fibroblasts that were quantified in the study were fibroblast growth factor (FGF), vascular endothelial growth factor (VEGF), trans-forming growth factor-beta1 and 2 (TGFβ1 and TGFβ2), and soluble collagen. A-PRF+ combined with fibroblasts demonstrated significantly higher values of released VEGF at every time point and, after 7 days, significantly higher values of released TGFβ2. A viability test after 72 h showed a significant increase in proliferation fibroblasts after exposition to the factors released from A-PRF+ combined with fibroblasts. Similarly, the degree of wound closure after 48 h was significantly higher for the factors released from A-RRF+ alone and the factors released from A-RRF+ combined with fibroblasts. These results imply that platelet-rich fibrin (PRF) enhanced with fibroblasts can be an alternative method of connective tissue transplantation. |
| |
| Keywords: | A-PRF+, fibroblast culture, wound healing, VEGF, TGFβ 2 |
|
|
