苯巴比妥诱导小鼠肝细胞增殖和阻断肝细胞凋亡的作用与小鼠肝内局部 …

Induction of hepatocyte proliferation and prevention of hepatocyte apoptosis by phenobarbital related to local humoral factor in mouse liver.

AIM: To study the association of phenobarbital (Phe) inducing hepatocyte proliferation and blocking hepatocyte apoptosis with local humoral factor in liver. METHODS: The ratio of liver/body weight, DNA content, regressive rate of hyperplastic liver, and DNA fragmentation were used to investigate whether the Phe-treated mouse liver extract (PMLE) and PMLE-95 (PMLE heated at 95 degrees C for 30 min) possessed Phe-like effects on mouse liver. Meantime, the effects of pretreatment with trypsin, RNAase, and DNAase on the activity of PMLE-95 were observed, and the differences of components between PMLE-95 and NMLE-95 (normal mouse liver extract, NMLE heated at 95 degrees C for 30 min) were analyzed with HPLC. RESULTS: PMLE-95 stimulated hepatocyte proliferation and prevented hepatocyte apoptosis caused by withdrawing Phe in mice, and the activity of PMLE-95 was eliminated after the pretreatment with trypsin. On the chromatograms PMLE-95 had 5 main peaks, while NMLE-95 had only 4 peaks. CONCLUSION: The effects of Phe on the liver were mediated by an intrinsic protein or peptide substance produced in response for the stimulation of Phe in mouse liver.