| 高效液相色谱法测定复方双花颗粒绿原酸的含量 |
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| 引用本文： | 凌海慧,刘峰群,韩晋,易毛,吴素体,胡琳.高效液相色谱法测定复方双花颗粒绿原酸的含量[J].解放军药学学报,2009,25(1):82-83. |
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| 作者姓名： | 凌海慧 刘峰群 韩晋 易毛 吴素体 胡琳 |
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| 作者单位： | 解放军第302医院,北京,100039 |
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| 摘 要： | 目的建立复方双花颗粒中绿原酸的含量测定方法。方法高效液相色谱法Phenomenex C18分析柱（5μm，4．6mm×250mm）；乙腈-0．4磷酸（10：90，三乙胺调pH值为3．0±0．2）为流动相；流速：1．0mL／min；检测波长327nm。结果绿原酸回归方程为Y=3621．370IX＋2．1458，r=0．9999（n=5），绿原酸浓度在12．0～28．0μg·mL^-1范围内呈线性关系，平均回收率为98．53％，RSD为0．57％。结论方法简便、准确、快速，可作为该制剂的定量方法。
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| 关 键 词： | 复方双花颗粒 HPLC 绿原酸 含量测定 |
Determination of Chlorogenic Acid in Fufangshuanghuakeli by HPLC |
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| LING Hai-Hui,LIU Feng-Qun,HAN Jin,YI Mao,WU Su-Ti,HU Lin.Determination of Chlorogenic Acid in Fufangshuanghuakeli by HPLC[J].Pharmaceutical Journal of Chinese People's Liberation Army,2009,25(1):82-83. |
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| Authors: | LING Hai-Hui LIU Feng-Qun HAN Jin YI Mao WU Su-Ti HU Lin |
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| Institution: | ( Hospital 302 of PLA, Beijing 100039 China) |
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| Abstract: | Aim To establish an assaying method of chlorogenic acid in Fufangshuanghuakeli. Methods HPLC Phenomenex C18 analytical column（5 μm ,4.6mm × 250mm） was used and acetonitrile-0.4 phosphonic acid （10： 90, pH value was adjusted to 3.0 ± 0.2 by triethylamine） was used as the mobile phase. The flow rate was 1.0mL/ rain and the detection wavelength was 327nm. Results Regression equation of chlorogenic acid was Y = 3 621. 370 1X ＋ 2. 145 8, r = 0. 999 9 （ n = 5 ）. It showed hnear correlation when concentration of chlorogenic acid ranged from 12.0μg·mL^-1 to 28.0μg·mL^-1. The average recovery was 98.53% and RSD was 0.57%. Conclusion This method is convenient, accurate and rapid, showing its potential to be used as a quantitative method of this preparation. |
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| Keywords: | HPLC |
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