| 高尔基体蛋白73联合甲胎蛋白异质体在原发性肝癌诊断中的价值 |
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| 引用本文： | 孔祥亘,韩绍磊,郑昭敏,杜文军,陈士俊.高尔基体蛋白73联合甲胎蛋白异质体在原发性肝癌诊断中的价值[J].山东大学学报(医学版),2011,49(4):110-114. |
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| 作者姓名： | 孔祥亘 韩绍磊 郑昭敏 杜文军 陈士俊 |
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| 作者单位： | 1. 山东大学医学院,济南,250012
2. 济南市传染病医院介入治疗中心,济南,250021 |
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| 基金项目： | 济南市科技发展计划项目 |
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| 摘 要： | 目的探讨高尔基体蛋白73(GP73)与甲胎蛋白异质体(AFP-L3)联合检测在乙型肝炎病毒相关性肝癌诊断中的价值。方法收集132例血清,其中原发性肝癌(PHC)56例、肝硬化33例,慢乙肝患者23例,健康对照20例,酶联免疫吸附法(ELISA)检测各组GP73的表达情况;应用甲胎蛋白异质体亲和吸附离心管洗脱获得AFP-L3,同时化学发光法检测原始血清中总AFP和AFP-L3,计算AFP-L3在总AFP中的比率。结果 PHC组、肝硬化组、肝炎组及健康对照组血清GP73水平分别为247.05(228.35～287.72)、176.83(159.51～183.98)、152.58(135.19～162.85)、43.00(37.96～52.64)ng/mL,AFP-L3%分别为14.52(12.33～15.28)%、5.68(4.32～7.74)%、4.31(3.57～6.45)%、3.29(2.60～4.25)%。PHC组GP73和AFP-L3%均明显高于其他组(P均<0.01);GP73和AFP-L3%用于诊断PHC的ROC曲线下面积分别为0.825、0.828,最佳截取值分别为187.60 ng/mL、9.98%,此时的敏感度分别为69.6%、64.3%,特异度分别为86.8%、96.1%。二者联合诊断PHC的敏感度为82.1%,特异度为85.5%。结论 GP73和AFP-L3%均可作为诊断PHC的血清标记物,二者联合检测具有更高的灵敏度,可提高PHC的诊断率。
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| 关 键 词： | 原发性肝癌 肿瘤标记物 高尔基体蛋白73 甲胎蛋白异质体 |
Values of Golgi protein 73 and Alpha-fetoprotein-L3 in the diagnosis of primary hepatic carcinoma |
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| KONG Xiang-gen,HAN Shao-lei,ZHENG Zhao-min,DU Wen-jun,CHEN Shi-jun.Values of Golgi protein 73 and Alpha-fetoprotein-L3 in the diagnosis of primary hepatic carcinoma[J].Journal of Shandong University:Health Sciences,2011,49(4):110-114. |
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| Authors: | KONG Xiang-gen HAN Shao-lei ZHENG Zhao-min DU Wen-jun CHEN Shi-jun |
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| Institution: | KONG Xiang-gen1,HAN Shao-lei2,ZHENG Zhao-min2,DU Wen-jun2,CHEN Shi-jun2(1.School of Medicine,Shandong University,Jinan 250012,China,2.Interventional Therapy Center,Jinan Infectious Hospital,Jinan 250021,China) |
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| Abstract: | Objective To explore significances of Golgi protein 73(GP73) and Alpha-fetoprotein variant(AFP-L3) in the diagnosis of hepatitis B virus-related primary hepatic carcinoma(PHC).Methods Enzyme-linked immunosorbent assay(ELISA) was used to detect the serum level of GP73 in 56 cases of PHC,33 cases of liver cirrhosis,23 cases of chronic hepatitis B and 20 healthy controls.AFP-L3 was isolated by using affinity microcentrifugalcolumn;AFP and AFP-L3 were detected with chemiluminescent immunoassay and then the prop... |
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| Keywords: | Primary hepatic carcinoma Tumor biomarker Golgi protein73 Alpha-fetoprotein variant |
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