决明毛状根化学成分研究

目的：研究决明毛状根的化学成分。方法：用发根农杆菌Agrobacterium rhizogenes 9402菌株从决明子叶外植体上诱导产生毛状根,在MS0液体培养基中大量培养获得。并采用色谱技术和波谱手段对毛状根的化学成分进行分离鉴定。结果：从决明毛状根95%乙醇提取物的醋酸乙酯部分分离鉴定了8个化合物,分别是白桦酯酸、大黄酚、大黄素甲醚、豆甾醇、1-羟基-7-甲氧基-3-甲基蒽醌、大黄酚-8-甲醚、大黄酚-1-甲醚和芦荟大黄素。其中芦荟大黄素是首次从决明毛状根中分得。结论：决明毛状根能合成与原植物相似的化学成分。

Studies on chemical constituents of hairy root of Cassia obtusifolia

Objective: To study the chemical constituents in hairy roots of Cassia obtusifolia.Method: The hairy roots of C.obtusifolia were induced with Agrobacterium rhizogenes LBA9402 from cotyledons and cultured in MS0 liquid medium.The compounds were isolated by silica gel,polyamide and Sephadex LH-20 column chromatography,and the structures were elucidated by employing chemical and spectral methods.Result: Eight compounds were isolated from the ethyl acetate fraction of 95% EtOH extract of the transformed roots of(C.obtusifolia).They are betulinic acid,chrysophanol,physcion,stigmasterol,1-hydroxy-7-methoxy-3-methyl-anthraquinone,8-O-methylchrysophanol,1-O-methylchrysophanol and aloe-emodin,and aloe-emodin was isolated from the hairy roots of C.obtusifolia for the first time.Conclusion: The hairy roots of C.obtusifolia have the ability to synthesize the similar chemical constituents as the original plants.