A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides |
| |
| Authors: | Velayos Antonio Fuentes-Vicente Mónica Aguilar-Elena Raúl Eslava Arturo P. Iturriaga Enrique A. |
| |
| Affiliation: | (1) Área de Genética, Departamento de Microbiología y Genética, Universidad de Salamanca, Edificio Departamental, Lab 301-2, Avenida Campo Charro s/n, 37007 Salamanca, Spain;(2) Neurogenetics Group, The Wellcome Trust Centre for Human Genetics, Roosevelt Drive, Oxford, OX3 7BN, UK;(3) Centro Hispano-Luso de Investigaciones Agrarias (CIALE), Universidad de Salamanca, Edificio Departamental, Lab 301-2, Avenida Campo Charro s/n, 37007 Salamanca, Spain |
| |
| Abstract: | ![]()
Two Mucor circinelloidesstructural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl diphosphate synthases. By functional complementation in Escherichia coli, the isoB gene product was shown to be a solanesyl diphosphate synthase (EC 2.5.1.11), which is the first fungal enzyme reported having this specificity. In addition, a M. circinelloides one-marker-per-chromosome map was completed by contour-clamped homogeneous electric field localisation of isoA, isoB and three other isoprenoid biosynthesis genes to individual chromosomes.Abbreviations FPP farnesyl diphosphate (or pyrophosphate) - GGPP geranylgeranyl diphosphate - PrenylPP prenyl diphosphate - DPP decaprenyl diphosphate - HPP hexaprenyl diphosphate - SPP solanesyl diphosphate |
| |
| Keywords: | Isoprenoid biosynthesis Carotenogenesis Mucor circinelloides FarnesylPP synthase SolanesylPP synthase |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
