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| 微波照射后小鼠睾丸损伤及凋亡的研究 | |
| 引用本文： | 季惠翔,宋波,张家华,余争平,李新,鄢俊安,沈文浩,李为兵. 微波照射后小鼠睾丸损伤及凋亡的研究[J]. 重庆医学, 2009, 38(21) |
| 作者姓名： | 季惠翔 宋波 张家华 余争平 李新 鄢俊安 沈文浩 李为兵 |
| 作者单位： | 第三军医大学西南医院泌尿外科,重庆,400038;第三军医大学预防医学系劳动卫生学教研室,重庆,400038 |
| 摘 要： | 目的 研究微波辐照对小鼠睾丸的损伤效应.方法 采用平均功率密度为65mW/cm2和90mW/cm2 微波辐照小鼠15min,采用HE染色和透射电镜方法观察小鼠睾丸组织的改变,用免疫组化方法观察计数睾丸内Caspase-3阳性细胞的表达情况.结果 微波急性辐照后12h,小鼠睾丸组织在光镜和电镜水平均出现损伤性改变,但65mW/cm2及90mW/cm2两个剂量组之间在形态学改变方面差异无统计学意义;辐照后0.5h Caspase-3蛋白阳性细胞数开始出现升高,24h达到高峰,其中90mW/cm2组升高幅度高于65mW/cm2组.结论 微波对小鼠睾丸组织存在明显损伤效应,并且能诱导凋亡相关因子Caspase-3表达增强,诱导效应存在着时效和量效依赖关系. |
| 关 键 词： | 微波 睾丸 损伤 |
Damaging and apoptosis effect of mice testis after microwave irradiation |
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| JI Hui-xiang,SONG Bo,ZHANG Jia-hua,et al.. Damaging and apoptosis effect of mice testis after microwave irradiation[J]. Chongqing Medical Journal, 2009, 38(21) | |
| Authors: | JI Hui-xiang SONG Bo ZHANG Jia-hua et al. |
| Affiliation: | JI Hui-xiang1,SONG Bo1,ZHANG Jia-hua1,et al.(1.Department of Urology,Southwest Hospital,Third Military Medical University,Chongqing 400038,China,2.Department of Occupation Health,Chongqing,400038,China) |
| Abstract: | Objective To investigate the damaging effect of mice testis exposed to microwave irradiation.Methods The mice were irradiated by microwave(mean power 90mW/cm2 or 65mW/cm2) for 15min respectively.The histological changes of mice testis was determined by HE stain and transmission electron microscope,and the expression of Caspase-3 protein was determined by immunohistochemistry.Results The damaging effect of testis exposed to microwave via transmission electron microscope was observed,and these damages reached... |
| Keywords: | microwave testis damage |
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