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Erythropoietin-induced proliferation of gastric mucosal cells |
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| Authors: | Itoh Kazuro Sawasaki Yoshio Takeuchi Kyoko Kato Shingo Imai Nobuhiro Kato Yoichiro Shibata Noriyuki Kobayashi Makio Moriguchi Yoshiyuki Higuchi Masato Ishihata Fumio Sudoh Yushi Miura Soichiro |
| Affiliation: | 1. Second Department of Internal Medicine, National Defense Medical College,Tokorozawa, Saitama, Japan 2. First Department of Anatomy, National Defense Medical College, Tokorozawa,Saitama, Japan 3. Department of Pathology, Tokyo Women's Medical College,Shinjuku-ku, Tokyo, Japan 4. Chugai Pharmaceutical Co., Ltd.,Gotenba, Shizuoka, Japan 5. Kisen Hospital, Katsushika-ku, Tokyo, Japan |
| Abstract: | AIM: To analyze the localization of erythropoietin receptor on gastric specimens and characterize the effects of erythropoietin on the normal gastric epithelial proliferation using a porcine gastric epithelial cell culture model. METHODS: Erythropoietin receptor was detected by RT-PCR, Western blotting and immunohistochermistry. Growth stimulation effects of erythropoietin on cultured gastric mucosal cells were determined by ELISA using bromodeoxyuridine (BrdU). RESULTS: Erythropoietin receptor was detected on cultured porcine gastric mucosal epithelial cells. Erythropoietin receptor was also detected histochemically at the base of gastric mucosal epithelium. BrdU assay demonstrated a dose-dependent increase in growth potential of cultured porcine gastric mucosal epithelial cells by administration of erythropoietin, as well as these effects were inhibited by administration of anti-erythropoietin antibody (P<0.01). CONCLUSION: These findings indicate that erythropoietin has a potential to proliferate gastric mucosal epithelium via erythropoietin receptor. |
| Keywords: | Erythropoietin Erythropoietin receptor Gastric epithelial cell proliferation Porcine gastric mucosal epithelial cells |
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