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| 体外培养兔角膜内皮细胞电生理特性的初步研究 | |
| 引用本文： | 张林,邢咏新,刘蓉,王玉英. 体外培养兔角膜内皮细胞电生理特性的初步研究[J]. 眼视光学杂志, 2008, 10(6) |
| 作者姓名： | 张林 邢咏新 刘蓉 王玉英 |
| 作者单位： | 1. 西安交通大学第一附属医院,眼科,陕西,西安,710061 2. 天津医科大学眼科临床学院,天津市眼科医院,天津,300070 3. 山西省大同医学院附属医院眼科,山西,大同,037008 4. 第四军医大学神经生物学系,陕西,西安,710032 |
| 基金项目： | 陕西省自然科学基金资助项目(Y100 323020):西安交通大学重点培植计划项目 |
| 摘 要： | 目的通过测定体外培养兔角膜内皮细胞(rabbitcorneal endothelial cells,RCECs)静息电位及全细胞电流,初步研究角膜内皮细胞的电生理特性。方法采用后弹力层及内皮层揭取联合组织块法进行RCECs体外培养,采用膜片钳全细胞记录模式记录原代及传代RCECs细胞静息电位及全细胞电流,绘制电流-电压(I/V)曲线。结果体外培养原代RCECs的平均静息膜电位水平为(20.9±1.7)mV(n=6),第2代RCECs的平均静息膜电位水平为(15.1±1.8)mV(n=6),两者差异有统计学意义(P<0.01)。在钳制电位+60mV下,原代RCECs全细胞电流为(396.2±39.0)pA(n=5),大于第2代RCECs的全细胞电流(201.4±45.2)pA(n=5),差异有非常显著的统计学意义(P<0.01)。结论后弹力层及内皮层揭取联合组织块法获得的原代及传代RCECs,在静息电位和全细胞电流等电生理特性方面存在差异。 |
| 关 键 词： | 角膜内皮细胞 细胞培养 膜片钳 细胞电生理 |
Electrophysiological characteristics of cul-tivated rabbit corneal endothelial cells |
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| ZHANG Lin,XING Yongxin,LIU Rong,et al.. Electrophysiological characteristics of cul-tivated rabbit corneal endothelial cells[J]. Chinese Journal of Optometry & Ophthalmology, 2008, 10(6) | |
| Authors: | ZHANG Lin XING Yongxin LIU Rong et al. |
| Affiliation: | ZHANG Lin,XING Yongxin,LIU Rong,et al. Department of Ophthalmology,the First Affiliated Hospital of the School of Medicine,Xi'an Jiaotong University,Xi'an China,710061 |
| Abstract: | Objective To investigate the characteristics of culti-vated rabbit corneal endothelial cells(RCECs)in vitro using a patch clamp.Methods RCECs were cultured to reveal Descement membrane and endothelium combining tissues masses.Whole-cell mode recordings were obtained from primary and secondary cultured RCECs.Resting potentials and whole-cell currents of RCECs were recorded and analyzed.Results Resting potentials and whole-cell currents of primary and secondary RCECs were(20.9±1.7)mV(n= 6),(15.1±1.8)mV(n=6)an... |
| Keywords: | corneal endothelial cells cell culture patch clamp cellular electrophysiological characteristics |
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