|
|||||
|
|
| 葡萄糖调节蛋白75基因对H2O2诱导的C6胶质瘤细胞氧化应激性损伤有保护作用 | |
| 引用本文： | 张莉莉,杨 柳. 葡萄糖调节蛋白75基因对H2O2诱导的C6胶质瘤细胞氧化应激性损伤有保护作用[J]. 南京医科大学学报(自然科学版), 2007, 27(12): 1366-1369 |
| 作者姓名： | 张莉莉 杨 柳 |
| 作者单位： | [1]南通大学护理学院,江苏南通226001 [2]南通大学附属医院神经外科,江苏南通226001 |
| 摘 要： | 目的:研究葡萄糖调节蛋白75(grp75)基因对H2O2诱导的C6胶质瘤细胞氧化应激性损伤的保护作用.方法:以grp75基因瞬时转染C6胶质瘤细胞,以H2O2作为氧化应激刺激物,建立细胞损伤模型,运用MTT、LDH、苔盼兰拒染、细胞周期流式细胞术(FCM)和凋亡小体方法观察grp75对H2O2引起的C6细胞氧化应激性损伤的影响.结果:grp75转染组C6细胞在H2O2(0.1 mmol/L,24 h)刺激后MTT增加、LDH活性降低,细胞凋亡明显抑制,与空载体转染组相比有显著差异.结论:grp75基因对H2O2引起的C6细胞氧化应激性损伤有保护作用. |
| 关 键 词： | 葡萄糖调节蛋白75基因 氧化应激 C6胶质瘤细胞 葡萄糖调节蛋白 基因对 胶质瘤细胞 氧化应激 应激性损伤 的保护 作用 glioma cells apoptosis 差异 载体 细胞凋亡 活性降低 刺激物 瞬时转染 结果 影响 方法观 凋亡小体 流式细胞术 |
| 文章编号： | 1007-4368（2007）12-1366-04 |
| 收稿时间： | 2007-05-08 |
| 修稿时间： | 2007-05-08 |
Protective effect of grp75 on H2O2-induced apoptosis of rat C6 glioma cells |
|
| ZHANG Li-li and YANG Liu. Protective effect of grp75 on H2O2-induced apoptosis of rat C6 glioma cells[J]. Acta Universitatis Medicinalis Nanjing, 2007, 27(12): 1366-1369 | |
| Authors: | ZHANG Li-li and YANG Liu |
| Affiliation: | Nursing College,Nantong University,Nantong 226001,Department of Neurosurgery,the Affiliated Hospital of Nantong University,Nantong 226001 |
| Abstract: | Objective:To investigate the protective effects of grp75 gene on hydrogen-peroxide(H2O2)-induced cell damage in rat glioma C6 cells. Methods:Cultured C6 glioma cells were transfected with grp75. Assays of MTT,LDH,FCM analysis and hoechst staining were used to investigate the protective effect of grp75 on H2O2 stimulated C6 cells. Results:Transfection of C6 glioma cells with grp75 resulted in the increased cell viability and decreased apoptosis ratio as compared to the control. Conclusion:These data indicated that grp75 might inhibit H2O2-mediated cytotoxic effect on C6 cells. |
| Keywords: | grp75 oxidative stress C6 glioma cells |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《南京医科大学学报(自然科学版)》浏览原始摘要信息 | |
| 点击此处可从《南京医科大学学报(自然科学版)》下载全文 | |