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| PKC在Ca2+信号调节MPF活性中的作用 | |
| 引用本文： | 刘莹,张杰,王亚杰,具英花,赵昕,宗志红,于秉治. PKC在Ca2+信号调节MPF活性中的作用[J]. 药物生物技术, 2003, 10(1): 14-16 |
| 作者姓名： | 刘莹 张杰 王亚杰 具英花 赵昕 宗志红 于秉治 |
| 作者单位： | 中国医科大学基础医学院生化教研室,沈阳,110001 |
| 基金项目： | 国家自然科学基金重点资助课题(39730460)，国家重点基础研究发展规划(973)项目(G-1999055900-2) |
| 摘 要： | 用Fura-2AM作为染料,用F-2000荧光分光光度计测细胞内Ca2+浓度的改变。用A23187-Ca2+载体提高细胞内Ca2+浓度,同时用PKC的特异性抑制剂calphostin C作用细胞来阻断PKC途径,观察MPF活性改变。结果表明:300nmol/L的Calphostin C可以最大限度地抑制PKC活性。Calphostin C不能影响A23187引起的细胞内Ca2+浓度的升高。A23187处理前加入300nmol/L光激活Calphostin C不能降低MPF的活性,而A23187单独却可以引起MPF的活性降低。与对照组相比,差异不显著,p<0.05。 |
| 关 键 词： | 调节 细胞内钙 蛋白激酶C M－期促进因子 细胞周期 |
| 文章编号： | 1005-8915(2003)01-0014-03 |
The Effects of PKC on Regulating MPF Activity by Intracellular Ca2+ Transient |
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| LIU Ying,ZHANG Jie,WANG Ya-jie,JU Ying-hua,ZHAO Xin,ZONG Zhi-hong,YU Bing-zhi. The Effects of PKC on Regulating MPF Activity by Intracellular Ca2+ Transient[J]. Pharmaceutical Biotechnology, 2003, 10(1): 14-16 | |
| Authors: | LIU Ying ZHANG Jie WANG Ya-jie JU Ying-hua ZHAO Xin ZONG Zhi-hong YU Bing-zhi |
| Abstract: | The activity of MPF can be regulated by intracellular Ca2+ transient, whether or not PKC has the effects in the way that Ca2+ transient regulate the activity of MPF. NIH3T3 cells was stained by Fura-2AM and detected the intracellular Ca2+ using F2000 spectrofluorometer . A23187 as a calcium ionophore can increase the intracellular Ca2+ . Calphostin C as PKC inhibitor can block the way of PKC. The experiment results showed that 300nmol/L calphostin C may inhibit the activity of PKC, but it hasn't effect the increase of intracellular Ca2+ induced by A23187. Intracellular Ca2+ transients can decreased the activity of MPF, and PKC may be involved in the MPF inactivity process. |
| Keywords: | Ca2+ PKC MPF |
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