Analysis and optimization of process parameters for in vitro biomineralization of CaCO3 by Klebsiella pneumoniae,isolated from a stalactite from the Sahastradhara cave

Stalactite is a speleothem which is usually made up of calcium carbonate crystals. In the present study the bacterial isolates, recovered from a stalactite from the Sahastradhara cave, were screened for their ability to precipitate calcium carbonate in order to understand whether mineralization in caves is a biogenic process or not. Five bacterial isolates were found to precipitate calcium carbonate via urease. The most potent bacterial isolate was identified as Klebsiella pneumoniae (accession number {"type":"entrez-nucleotide","attrs":{"text":"MG946801","term_id":"1578806359","term_text":"MG946801"}}MG946801) based on 16S rDNA sequencing. The optimized conditions, for calcium carbonate precipitation, determined by response surface methodology using CCD were found to be: 1.5625% urea, 19.98% inoculum level, 6.98 pH and 38 h 24 min. The morphology and crystalline structure of the precipitated mineral were revealed by SEM. EDX analysis confirmed the presence of carbon, oxygen and calcium in a precipitated crystal. XRD analysis confirmed the crystalline structure of a mineral with rhombohedral shape and 166 Å crystal size. This bacterium can serve as a promising candidate for producing bioconcrete.

Stalactite is a speleothem which is usually made up of calcium carbonate crystals.