抗环瓜氨酸肽抗体与葡萄糖6磷酸异构酶抗原联合检测在类风湿性关节炎诊断中的应用

目的探讨抗环瓜氨酸肽（CCP）抗体和葡萄糖6磷酸异构酶（GPI）抗原联合检测对类风湿性关节炎（RA）的诊断意义。方法对120例（RA67例,非RA53例）患者血清同时进行抗CCP抗体、类风湿因子（RF）和GPI抗原检测,检测方法分别为酶联免疫吸附试验（ELISA）、胶乳增强免疫透射比浊法。结果在RA组中,3个指标的阳性率均显著高于非RA组（P〈0.001）;抗CCP抗体和GPI抗原联合检测与RF存在显著相关性（r=0.48,P〈0.05）,且联合检测的敏感性（79.1%）显著高于单独检测RF（76.1%）,受试者工作特征（ROC）曲线下的面积（AUG）CCP/GPI=0.903,均显著大于AUGCCP、AUGGPI、AUGRF（P〈0.05）。结论联合应用抗CCP抗体与GPI抗原显著提高RA的诊断效率,是诊断RA的理想指标。

Application of the combined detection of anti-cyclic citrullinated peptide antibody and glucose-6-phosphate isomerase antigen in the diagnosis of rheumatoid arthritis

Objective To investigate the diagnostic significance of the combined detection of anti-cyclic citrullinated peptide（CCP） antibody and glucose-6-phosphate isomerase（GPI） antigen in the diagnosis of rheumatoid arthritis（RA）.Methods The serum anti-CCP antibody,rheumatoid factor（RF） and GPI antigen were determined in 120 patients（67 RA and 53 non-RA） by enzyme-linked immunosorbent assay（ELISA） and latex immunoenhancement nephelometry.Results In RA group,the positive rates of the three indexes were significantly higher than those in non-RA group（P0.001）.There was a correlation between RF and the combined detection of anti-CCP antibody and GPI antigen（r=0.48,P0.05）.The sensitivity of combined detection（79.1%） was significantly higher than that of individual detection of RF（76.1%）,and the area under receiver operating characteristic（ROC） curve（AUG）CCP/GPI（0.903） was larger than AUGCCP,AUGGPI and AUGRF（P0.05）.Conclusions The combined application of anti-CCP antibody and GPI antigen improves the diagnosis efficiency of RA.It is an ideal index to the diagnosis of RA.