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| 体外培养鼠破骨细胞对牙体硬组织的吸收实验 | |
| 引用本文： | 张庆鸿,梁星,刘梦桃. 体外培养鼠破骨细胞对牙体硬组织的吸收实验[J]. 口腔医学, 2007, 27(9): 452-454,468 |
| 作者姓名： | 张庆鸿 梁星 刘梦桃 |
| 作者单位： | 浙江大学医学院附属口腔医院修复科,杭州,310006;四川大学华西口腔医院修复科,成都,610041;浙江省杭州哼哈口腔医院修复科,杭州,310006 |
| 基金项目： | 国家自然科学基金资助项目（30271428） |
| 摘 要： | 目的本文旨在观察体外培养鼠破骨细胞对牙体硬组织的吸收。方法采用鼠长骨机械分离法获得破骨细胞,通过破骨细胞形态学观察及其特异性染色鉴定后,在牙本质上培养破骨细胞并用扫描电镜观察其吸收情况。结果鼠破骨细胞能在牙本质磨片上形成大小不一、形态不规则的吸收陷窝。结论长骨机械分离法可获得一定量特异性染色阳性的破骨细胞,并可在牙本质磨片上形成明显吸收陷窝。 |
| 关 键 词： | 破骨细胞 吸收 硬组织 鼠 |
| 文章编号： | 1003-9872（2007）09-0452-04 |
| 修稿时间： | 2007-02-15 |
Resorption of rat osteoclasts on dentine slices in vitro culture |
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| ZHANG Qing-hong,LIANG Xing,LIU Meng-tao. Resorption of rat osteoclasts on dentine slices in vitro culture[J]. Stomatology, 2007, 27(9): 452-454,468 | |
| Authors: | ZHANG Qing-hong LIANG Xing LIU Meng-tao |
| Affiliation: | Department of Prosthetics, Aff-diated Stomatology Hospital, College of Medical Sciences, Zhejiang University, Hangzhou 310006, China |
| Abstract: | Objective To observe resorption of rat osteoclasts on dentine.Methods Rat osteoclasts were obtained through anatomic long-bone technique.After the osteoclasts were confirmed through morphology and special dyeing,they were cultured on dentine and their absorption lacunae were observed through SEM.Results Rat osteoclasts could excavate irregular lacunae on dentine.Conclusion Anatomic long-bone technique could provide a certain amount of osteoclasts,which were positive to special dyeing and could excavate lacuna on dentine. |
| Keywords: | osteoclast resorption sclerous tissues rats |
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