| 温州汉族人群TH01和TPOX基因座的遗传多态性研究 |
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| 引用本文： | 张洪勤,应俊,李佩珍,吴淑珍. 温州汉族人群TH01和TPOX基因座的遗传多态性研究[J]. 中国优生与遗传杂志, 2008, 16(6): 23-24 |
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| 作者姓名： | 张洪勤 应俊 李佩珍 吴淑珍 |
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| 作者单位： | 1. 温州医学院生物实验教学中心
2. 温州医学院基础学院,浙江温州,325027 |
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| 摘 要： | 目的研究STR位点TH01和TPOX在温州汉族人群的遗传多态性,获得相应的群体遗传学数据。方法EDTA抗凝血样采自温州197名无血缘关系汉族个体,用chelex-100法提取DNA,PCR扩增,聚丙烯酰胺凝胶电泳,银染显色分析。结果TH01观察到6个等位基因及16种基因型;TPOX观察到5个等位基因及12种基因型。各基因座的杂合度（H）分别为0.659和0.619;非父排除率（PE）分别为0.367和0.315;个人识别能力（Dp）分别为0.839和0.766。结论TH01和TPOX基因座在温州地区汉族群体中具有较高杂合度,等位基因分布符合Hardy-Weinberg平衡,在法医学应用和群体遗传学研究中有较高的价值。
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| 关 键 词： | 短串联重复序列 聚合酶链反应 遗传多态性 |
| 文章编号： | 1006-9534(2008)06-0023-02 |
| 修稿时间： | 2007-11-21 |
A study of genetic polymorphism of the STR locus TH01 and TPOX in Han population in Wenzhou |
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| ZHANG Hong-qin. A study of genetic polymorphism of the STR locus TH01 and TPOX in Han population in Wenzhou[J]. Chinese Journal of Birth Health & Heredity, 2008, 16(6): 23-24 |
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| Authors: | ZHANG Hong-qin |
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| Affiliation: | ZHANG Hong - qin, et aL (Molecular Biology Research Centre of Wenzhou Medical College, Wenzhou 325027) |
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| Abstract: | Objective： To study genetic polymorphism of TH01 and TPOX in Han population in Wenzhou. Methods： DNA was extracted with chelex - 100 method from EDTA - blood samples of 197 unrelated individuals in Wenzhou and amplified with PCR technique. The PCR products were analyzed by PAG vertical electrophoresis and silver - stain. Results： 6 alleles and 16 genotypes of TH01, 5 alleles and 12 genotypes of TPOXwas observed. The heterozygosities of TH01 and TPOX are 0. 659 and 0. 619 ; The power of exclusion are 0. 367 and 0. 315 ; The power of discriminating are 0. 839 and 0. 766 . Conclusion： The heterozygosities of TH01 and TPOX are high and the frequencies was in accordance with Hardy - Weinberg equilibrium （ P 〉 0. 05）, so the three loci can be used in forensic medicine and in other genetic researches. |
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| Keywords: | Short tandem repeats Polymerase chain reaction Genetic polymorphism |
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