参芪扶正注射液对肝癌细胞株H22的抑制作用及机制研究

目的研究参芪扶正注射液体内外对肝癌H22细胞的抑制作用及其机制。方法 MTT法检测参芪扶正注射液体外对H22细胞增殖的影响;利用H22小鼠模型进行体内抗肿瘤作用试验,计算抑瘤率;酶联免疫ELISA法测荷瘤小鼠血清IFN-γ含量;双抗体夹心ABC-ELSA法测荷瘤小鼠血清TNF-α的含量。结果参芪扶正注射液体内外均能抑制H22细胞的生长,具有浓度和时间依赖性;促进荷瘤小鼠血清中IFN-γ和TNF-α的表达。结论参芪扶正注射液体内外均能抑制肝癌H22的生长,其抗肿瘤作用与调节荷瘤小鼠的免疫功能有关。

Anti-tumor effect and mechanism of Shenqifuzheng injection on hepatocellular carcinoma cell line H22

Objective To study the inhibition effect and mechanism on H22 of Shenqifuzheng injection.Methods MTT was performed to observe the proliferation of H22 cell in vitro;then anticancer experiment in vivo was done by H22 mice model,inhibitory rate was calculated;the serum content of IFN-γ was measured by ELISA assay;the content of TNF-α by enzyme-linked immunosorbent assay. Results Shenqifuzheng injection inhibited the proliferation of H22 cells in a concentration and timedependent manner,promoted the expression of IFN-γ and TNF-α in the serum of tumor-bearing mice. Conclusion Shenqifuzheng injection can inhibit the growth of H22 in vitro and in vivo,the anti-tumor effect related with the immune function of tumor-bearing mice improved.