生地黄化学成分的分离与鉴定

目的对生地黄的化学成分进行研究。方法运用硅胶柱色谱、Sephadex LH-20柱色谱、制备HPLC等分离手段对生地黄95%乙醇提取物乙酸乙酯萃取层进行化学成分的分离纯化,根据理化性质及波谱数据鉴定其结构。结果分离得到10个化合物,分别鉴定为7-羟基异喹啉(7-isoquinoli-nol,1)、5-羟基-2-羟甲基吡啶(5-hydroxy-2-pyridinemethanol,2)、6-甲基-3-吡啶醇(6-methyl-3-pyr-idinol,3)、红景天苷(salidroside,4)、地黄苷C(rehmaionoside C,5)、地黄苷A(rehmaionoside A,6)、腺嘌呤核苷(adenosine,7)、腺嘌呤(adenine,8)、尿嘧啶核苷(uridine,9)及β-谷甾醇(β-sitosterol,10)[1]。结论化合物1-4为首次从地黄属植物中分离得到。

Isolation and identification of the chemical constituents from Rehmannia glutinosa L.

Objective To study the chemical constituents from Rehmannia glutinosa. Methods Compounds were isolated by chromatography on silica gel, Sephadex LH-20 and PHPLC. Their structures were confirmed on the basis of physico-chemical constants and spectroscopic analysis. Results Ten compounds were isolated and their structures were identified as 7-isoquinolinol (1), 5-hydroxy-2-pyridinemethanol (2), 6-methyl-3-pyridinol (3), salidroside (4), rehmanniosid C (5), rehmannioside A (6), adenosine（7）, adenine（8）, uridine（9）and β-sitosterol（10）. Conclusion Compounds 1-4 are isolated from Rehmannia genus for the first time.