白细胞介素-6基因多态性与冠心病的相关性研究及其对血脂的影响

目的 探讨白细胞介素6（IL-6） 基因启动子区基因-572位点和-634位点多态性与冠心病的关系,及其对血脂、脂蛋白、载脂蛋白水平的影响.方法 采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法,检测165例冠心病（冠心病组）患者和170名健康人（对照组） 的IL-6基因型;按常规方法测定血脂、脂蛋白、载脂蛋白水平.结果 冠心病组总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）水平均明显高于对照组（P均〈0.05）;IL-6基因-634位点多态性在冠心病组和对照组的分布差异无显著性（P〉0.05）,而IL-6基因-572位点多态性在两组人群中的分布差异存在显著性（P〈0.05）;等位基因频率的相对风险分析发现,G等位基因携带者患冠心病的风险是C等位基因的1.652倍相对比值比（OR）=1.652,95%可信区间（CI）：1.137～2.401],携带G等位基因的冠心病个体血清TC水平显著高于不携带者（P〈0.05）.结论 IL-6基因-572位点多态性与冠心病的发病具有相关性,其中G等位基因是冠心病重要的遗传标记;IL-6基因-572位点多态性可能通过影响血脂水平而影响冠心病的发生.

Relationship between interleukin-6 gene polymorphism and coronary heart disease and its effect on plasma lipid levels

OBJECTIVE: To study the relationship between interleukin-6 (IL-6) gene promoter-572 C/G and -634 C/G polymorphism and coronary heart disease (CHD) and the influence of IL-6 gene polymorphism on plasma lipid and lipoprotein levels. METHODS: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used for the detection of IL-6 genotype in 165 patients with CHD and 170 healthy persons. The plasma lipid and lipoprotein levels were measured by routine method. RESULTS: Plasma total cholesterol (TC), triglyceride (TG) and low density lipoprotein cholesterol (LDL-C) of the patients with CHD were significantly higher than those of the controls (all P<0.05). The distributions of IL-6 gene -634 C/G polymorphism were not different between CHD group and control group (P>0.05), but the IL-6 gene-572 C/G polymorphism was significantly different between them (P<0.05). The relative risk of CHD of C allele was 1.652 times to the G allele odds ratio (OR)=1.652, 95% confidence interval (CI): 1.137-2.401]. The level of plasma lipid in G allele carriers was significantly higher than non-carriers (P<0.05). CONCLUSION: IL-6 gene -572 C/G polymorphism is associated with CHD, and G allele is an important genetic marker. IL-6 gene polymorphism may affect CHD through elevation of plasma lipid and lipoprotein levels.