生物反应器在构建组织工程产品中的应用研究

目的：通过对4种生物反应器的介绍，分析不同生物反应器在细胞培养中的作用与用途。方法：观察不同生物反应器对细胞进行培养的结果。结果：不同类型的生物反应器及其机械性能、传质以及流体剪应力等对细胞培养组织的形态和功能产生的影响不同。结论：旋转壁式生物反应器更接近于三维悬浮生长和生物体的内部环境。将来的生物反应器将是集培养、控制、研究、生产于一体的智能化系统生命机器，也是生物反应器发展的最终目标。

Application and Research of Bioreactor in Developing Products of Tissue Engineering

Objective To analyze the effects and applications of 4 kinds of bioreactors on cell culture. Methods The results of different bioreactors for cell culture were observed. Results The bioreactors with different mechanical properties, mass transfer and fluid shear stress had different impacts on morphological and functional organizations. Conclusion Rotating bioreactor is more suitable for three-dimensional suspension growth and similar to organisms internal environment than the others. It is indicated that bioreactors will be integrated intelligent devices including culture, control, research, and production in the future.