白英水提物诱导人卵巢癌A2780细胞bcl-2基因表达的影响

目的：观察白英水提物诱导人卵巢癌A2780细胞凋亡的作用，初步探讨其分子机制。方法：常规方法制备白英水提物，体外培养人卵巢癌A2780细胞，MTT法提示其有较强的体外抑制A2780细胞作用。采用半定量RT—PCR法检测凋亡相关基因bcl-2表达。实验组设白英水提物12．5、25．0、50．0mg／ml 3种浓度，以顺铂（25．0μg／ml）为阳性对照组。结果：白英水提物对A2780细胞增殖有抑制作用，且呈明显的量效关系，半定量RT—PCR法检测显示bcl-2 mRNA表达下调，诱导其发生凋亡。结论：白英水提物对A2780细胞有较强的增殖抑制和诱导凋亡作用，其诱导A2780细胞凋亡的分子机制可能与下调bcl-2基因表达有关。

Apoptosis-induced effect of solanum lyratum thunb extract on human cancer A2780 cells

Objective： To investigate the proliferation inhibitory and apoptosis - inducing effect of solanum lyratum thunb extract and its molecular mechanism on human cancer A2780 ceils. Methods： Dried whole herbs of solanum lyratum thunb were extracted by boiling distilled water. Human cancer A2780 cells were randomly divided into the control group, solanum lyratum thunb treated groups （12.5,25.0, 50.0 mg/ml） and DDP （25.0 mg/L） group. The expression of Bcl - 2 mRNA were detected by semi - quantitative RT - PCR. Results： Solanum lyratum thunb extract displayed a strong proliferation inhibitory effect in a dose dependent manner in A2780 cells. Bcl -2 mRNA levels were down -regulated. Conclusion： Solanum lyratum thunb extract displays a strong proliferation inhibitory and apoptosis - inducing effect in a dose - and - time - dependent manner in A2780 cells. The molecular mechanism of apoptosis of A2780 cells induced by solanum lyratum thunb extract may be related to the down - regulation of bcl- 2 gene.