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| 腺相关病毒介导的肝细胞生长因子Kringle 1结构域对人前列腺癌裸鼠骨移植瘤生长的影响 | |
| 引用本文： | 刘增军,沈赞,赵晖,杜雪菲,丰涛,李洪涛,林李家宓,姚阳. 腺相关病毒介导的肝细胞生长因子Kringle 1结构域对人前列腺癌裸鼠骨移植瘤生长的影响[J]. 肿瘤, 2011, 31(7): 585-590. DOI: 10.3781/j.issn.1000-7431.2011.07.003 |
| 作者姓名： | 刘增军 沈赞 赵晖 杜雪菲 丰涛 李洪涛 林李家宓 姚阳 |
| 作者单位： | 1. 上海交通大学附属第六人民医院肿瘤内科,上海,200233 2. 香港中文大学脑肿瘤研究所,香港,999077 |
| 基金项目： | 国家自然科学基金资助项目(编号:30872991)上海市科学技术委员会科研计划项目 |
| 摘 要： | 目的:探讨肝细胞生长因子Kringle1结构域(kringle1domain of hepatocyte growth factor,HGFK1)基因对人前列腺癌裸鼠骨移植瘤生长的影响。方法:通过向裸鼠胫骨骨髓腔内注射人前列腺癌PC-3细胞,建立人前列腺癌裸鼠骨移植瘤模型;分别向移植瘤和裸鼠尾静脉内注射PBS、携带有HGFK1基因或增强型绿色萤光蛋白(enhanced green fluorescent protein,EGFP)基因的腺相关病毒载体的病毒上清液rAAV-EGFP和rAAV-HGFK1,观察肿瘤生长、体内转移和裸鼠生存时间,免疫组织化学法检查移植瘤组织中Ki67、E-cadherin和CD31的表达。结果:成功建立了人前列腺癌裸鼠骨移植瘤模型,rAAV-HGFK1组的肿瘤体积和质量明显小于PBS和rAAV-EGFP组(P<0.05),抑瘤率达46.69%;rAAV-HGFK1组裸鼠的中位生存时间比PBS和rAAV-EGFP组明显延长(P<0.05);PBS和AAV-EGFP组腹主动脉旁淋巴结转移发生率高于rAAV-HGFK1组(P<0.05);rAAV-HGFK1组肿瘤组织中Ki67和CD31的表达明显低于PBS和AAV-EGFP组(P<0.05),E-cadherin的表达明显高于PBS和AAV-EGFP组(P<0.05)。结论:HGFK1具有抑制人前列腺癌裸鼠骨移植瘤生长、转移和血管生成的作用,并可延长荷瘤裸鼠的生存时间。 |
| 关 键 词： | 前列腺肿瘤 疾病模型,动物 新生血管化,病理性 肝细胞生长因子Kringle 1结构域 |
Effect of adenovirus-associated virus carrying kringle 1 domain of hepatocyte growth factor gene on the growth of human prostatic carcinoma xenograft in nude mouse bone |
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| LIU Zeng-jun,SHEN Zan,ZHAO Hui,DU Xue-fei,FENG Tao,LI Hong-tao,LIN-LI Jia-mi,YAO Yang. Effect of adenovirus-associated virus carrying kringle 1 domain of hepatocyte growth factor gene on the growth of human prostatic carcinoma xenograft in nude mouse bone[J]. Tumor, 2011, 31(7): 585-590. DOI: 10.3781/j.issn.1000-7431.2011.07.003 | |
| Authors: | LIU Zeng-jun SHEN Zan ZHAO Hui DU Xue-fei FENG Tao LI Hong-tao LIN-LI Jia-mi YAO Yang |
| Affiliation: | LIU Zeng-jun1,SHEN Zan1,ZHAO Hui1,DU Xue-fei1,FENG Tao1,LI Hong-tao1,LIN-LI Jia-mi2,YAO Yang1 1. Department of Oncology,Sixth People's Hospital,Shanghai Jiaotong University,Shanghai 200223,China,2. Brain Tumor Institute,Chinese University of HongKong,Hong Kong 999077 |
| Abstract: | Objective:To observe the effect of adenovirus-associated virus carrying kringle 1 domain of hepatocyte growth factor(HGFK1) gene on the growth of human prostatic carcinoma xenograft in nude mouse bone. Methods:The human prostatic carcinoma xenograft in nude mouse bone was established by injection of PC-3 cells into the mouse tibial bone marrow cavity. PBS or the supernatant of recombinant adenovirus-associated virus carrying enhanced green fluorescent protein(EGFP) gene(rAAV-EGFP) or HGFK1 gene(rAAV-HGFK1) ... |
| Keywords: | Prostatic neoplasms Disease models animal Neovascularization pathologic Kringle 1 domain of hepatocyte growth factor |
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