Comparison of polyclonal and monoclonal antibodies to Actinomyces and Arachnia species |
| |
| Authors: | RISTO-PEKKA HAPPONEN,PERTTI ARSTILA,MARKKU VIANDER,EVA SÖ DERLING,MATTI VILJANKN |
| |
| Affiliation: | Institute of Dentistry, University of Turku, Turku, Finland;Department of Virology, University of Turku, Turku, Finland;Department of Medical Microbiology, University of Turku, Turku, Finland |
| |
| Abstract: | ![]()
Abstract – Polyclonal (PoAbs) and monoclonal (MoAbs) antibodies were produced to Actinomyces israelii serotypes 1 and 2, to Actinomyces naeslundii, and to Arachnia propionica, and their specificities were studied by an enzyme immunoassay (EIA). All PoAbs except those to A. propionica reacted also with at least one other Actinomyces species. Only the MoAb to A. naeslundii proved to be more specific than the corresponding PoAbs. This MoAb did not cross-react with other Actinomyces or Arachnia species, nor with any other anaerobic or aerobic bacteria studied by inhibition EIA. Immunoblotting studies indicated that the antibody specific to A. naeslundii is directed against a large molecular weight antigen (>150 kd), probably polysaccharide in nature. The produced PoAbs and MoAbs can be used for further analyses of the antigenic determinants of different Actinomyces and Arachnia species. |
| |
| Keywords: | Actinomyces antibodies, monoclonal determinants, antigenic enzyme-linked immunosorbent assay immunoblotting |
|
|
