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| 经济、有效提取外周血基因组DNA的方法 | |
| 引用本文： | 白春英,周静,瑞云,于晓明,萨初然贵,李秀君.经济、有效提取外周血基因组DNA的方法[J].检验医学与临床,2010,7(17):1795-1795,1798. |
| 作者姓名： | 白春英 周静 瑞云 于晓明 萨初然贵 李秀君 |
| 作者单位： | 1. 内蒙古赤峰学院医学院,024000 2. 内蒙古赤峰学院第一附属医院儿科,024000 3. 内蒙古赤峰市医院儿科,024000 |
| 摘 要： | 目的介绍一种经济、有效提取外周血基因组DNA的方法。方法采用0.2%NaCl处理人外周血并收集白细胞,再用基因组DNA提取试剂盒抽提基因组DNA。结果用原本提取100μL全血基因组DNA的试剂量来提取3mL全血的基因组DNA,费用节省了2倍以上。结论该方法方便、有效,同时还能得到大量的基因组DNA。 |
| 关 键 词： | 经济 有效 提取 外周血基因组DNA |
An economical and effective method of extracting peripheral blood genomic DNA |
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| BAI Chun-ying,ZHOU Jing,RUI Yun,YU Xiao-ming,SACHU Ran-gui,LI Xiu-jun.An economical and effective method of extracting peripheral blood genomic DNA[J].Laboratory Medicine and Clinic,2010,7(17):1795-1795,1798. | |
| Authors: | BAI Chun-ying ZHOU Jing RUI Yun YU Xiao-ming SACHU Ran-gui LI Xiu-jun |
| Institution: | BAI Chun-ying1,ZHOU Jing1,RUI Yun1,YU Xiao-ming2,SACHU Ran-gui3,LI Xiu-jun1.1.School of Medical Science of Chifeng College,Inner Mongolia 024000,China,2.Department of Pediatrics,First Affiliated Hospital of Chifeng College,3.Department of Pediatrics the Hospital of Chifeng City,Inner Mongolia,024000 |
| Abstract: | Objective To introduce an economical and effective method for extracting human peripheral blood genomic DNA.Methods The leukocytes were isolated from human peripheral blood by 0.2% NaCl and then the genomic DNA was extracted from the leukocytes with a DNA extract kit.Results The reagents volume of extract 100 μL peripheral blood genomic DNA by original method could extract 3mL peripheral blood genomic DNA by new method,with the cost being saved by more than two-fold.Conclusion This method is very economical and effective,meanwhile it can also obtain a large number of genomic DNA. |
| Keywords: | economic effective extract peripheral blood genomic DNA |
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