聚合酶链反应在结核病诊断中的价值 |
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引用本文: | 易健华,曾发宗,胡望福,刘春兰. 聚合酶链反应在结核病诊断中的价值[J]. 井冈山医专学报, 1999, 6(2): 24-25,27 |
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作者姓名: | 易健华 曾发宗 胡望福 刘春兰 |
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作者单位: | [1]吉安地区卫生防疫站,吉安市343000 [2]吉安地区结防所,吉安市3543000 |
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摘 要: | 目的:为早期诊断结核病寻找一种简便、快速、灵敏的实验室方法。方法:用PCR、结核菌培养、涂片法对临床可疑结核病患者进行检验。结果:在16份痰标本和其它标本中,PCR、结核菌培养、涂片的阳性检出率分别为56.9%、20.7%和7.7%。结论:PCR与其它两种方法比较,差异均非常显著,揭示PCR对结核病的确诊有重要辅助意义。
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关 键 词: | 聚合酶链反应 结核病 诊断 PCR 痰标本 |
The value of Polymerase chain reachion in diagnosing tuberculosis |
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Affiliation: | (Yi Jianhua etal the Sanitation and antiepidemic station of Jian Prefcture,Jiangxi,343000) |
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Abstract: | Objective: To search for a simple and convenient, fast and sensihve laboratory methodon diagnosing tubereulosis earlier. Methods: Test for Mycobacterium tuberculosis by polymerase chainreaction(PCR), culture of bacteria and smear in the dubious tuberculars of clinical diagnosis. Results:detection rates of PCR, culture and smear were 56. 9%, 20. 7 % and 7. 7 % in 16 units of phlegm and theother sampoes. Conclusion: There were all significantly differences in comparing PCR with the othermethods. It indicates that PCR is an important and significative supplementary method to diagnose tu-bereulosis. |
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Keywords: | tuberculosis Polymerase chain reaction(PCR) diagnosis |
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