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| 小鼠精子发生过程中dysbindin-1的表达 | |
| 引用本文： | 闫慧刘慧霞魏宗波郭蕊张涛 冯雅琴. 小鼠精子发生过程中dysbindin-1的表达[J]. 解剖学报, 2015, 46(4): 548-552. DOI: 10.16098/j.issn.0529-1356.2015.04.018 |
| 作者姓名： | 闫慧刘慧霞魏宗波郭蕊张涛 冯雅琴 |
| 作者单位： | 1. 山西医科大学组织学胚胎学教研室，太原 030001; 2. 中国科学院遗传与发育生物学研究所，北京 100101 |
| 基金项目： | dysbindin在精子顶体形成中的作用;dysbindin在小鼠睾丸顶体形成细胞中的表达;dysbindin在精子顶体形成中的作用 |
| 摘 要： | 目的 探讨精子发生过程中dysbindin-1的表达及dysbindin-1对精子顶体形态的影响。 方法 取生后7d、14d、21d、28d、35d和3月龄的雄性小鼠各3只，用免疫印迹法检测睾丸组织dysbindin-1的表达；以dysbindin-1缺失突变的sdy小鼠为研究对象，收集附睾尾部精子，一部分制备精子涂片，HE染色显示精子形态，用异硫氰酸荧光素-豌豆凝集素（FITC-PSA）和抗精子蛋白56（sp56）单克隆抗体进行荧光染色显示顶体的结构；另一部分采用免疫印迹法检测精子中dysbindin-1的表达。 结果 不同发育阶段小鼠睾丸组织及精子中均只有dysbindin-1A，无dysbindin-1C的表达；sdy小鼠的精子及顶体的形态未见明显异常。 结论 Dysbindin-1A表达于小鼠精子发生的不同时期，dysbindin-1A对精子形态维持不起关键作用。 |
| 关 键 词： | Dysbindin-1 睾丸 精子 顶体 免疫印迹法 sdy 小鼠 小鼠 |
| 收稿时间： | 2014-11-04 |
| 修稿时间： | 2015-01-04 |
Expression of dysbindin-1 during spermatogenesis |
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| YAN Hui LIU Hui-xia WEI Zong-bo GUO Rui ZHANG Tao FENG Ya-qin. Expression of dysbindin-1 during spermatogenesis[J]. Acta Anatomica Sinica, 2015, 46(4): 548-552. DOI: 10.16098/j.issn.0529-1356.2015.04.018 | |
| Authors: | YAN Hui LIU Hui-xia WEI Zong-bo GUO Rui ZHANG Tao FENG Ya-qin |
| Affiliation: | 1. Department of Histology and Embryology, Shanxi Medical University, Taiyuan 030001,China; 2. Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101，China |
| Abstract: | Objective To test dysbindin-1 expression during spermatogenesis and dysbindin-1 effect on histology of acrosome in mice. Methods Western blotting analysis was applied to detect the expression of dysbindin-1 in testis on day 7,14,21,28 and 35 postpartum and three-month old mice(n=3). The caudal spermatozoa was collected from sdy mice (no dysbindin-1expression), HE staining was performed for sperm morphology, fluorescein isothiocyanate conjugate-pisum sativum agglutinin (FITC-PSA) staining and immunofluorescence staining with antibody against sperm protein 56(sp56) for acrosome on sperm smear. Results Dysbindin-1A, not -1C, was expressed in postnatal testis development and spermatozoa. Mature sperm from sdy caudal epididymis had normal formation. Conclusion Dysbindin-1A, expressed in testis tissue and sperm during spermatogenesis, is not required for maintaining the morphology of sperm. |
| Keywords: | Dysbindin-1 Testis Sperm Acrosome Western blotting sdy mouse Mouse |
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