益肾健脾泄浊汤对痛风性肾病模型大鼠肾功能及肾脏病理改变的影响*

目的探讨益肾健脾泄浊汤对痛风性肾病模型大鼠肾功能指标及肾脏病理改变的影响。方法 将60只SD雄性大鼠随机分为：空白组、模型组、阳性对照组（非布司他片组）、益肾健脾泄浊汤高、中、低剂量组。采用高酵母粉联合腺嘌呤灌胃的方法复制痛风性肾病模型大鼠。造模成功后，分别按照非布司他片组（3.6 mg/kg/d），益肾健脾泄浊汤高、中、低剂量剂量组（中药浸膏5.86 g/kg/d + 三七粉0.54 g/kg/d、中药浸膏2.93 g/kg/d + 三七粉0.27 g/kg/d、中药浸膏1.47 g/kg/d + 三七粉0.14 g/kg/d）灌胃给药，连续干预21 d后检测大鼠的血尿酸（serum uric acid，SUA）、血肌酐（serum creatinine，SCr）和血尿素氮（blood urea nitrogen，BUN）水平，计算其肾脏指数并观察肾脏的病理改变情况。结果 与空白组比较，模型组大鼠SUA、SCr、BUN显著升高（P<0.05），肾脏病理改变比较明显；与模型组相比较，益肾健脾泄浊汤高、中、低剂量组大鼠SUA、BUN水平均有下降（P<0.05），益肾健脾泄浊汤高、中剂量组大鼠SCr水平显著下降（P<0.05），低剂量组大鼠SCr水平有下降趋势，但差异无统计学意义，且各组大鼠肾脏病理改变均有不同程度的改善。结论 益肾健脾泄浊汤可降低痛风性肾病模型大鼠的SUA、BUN、SCr水平，改善其肾脏病理损伤，保护大鼠肾脏功能。

Effect of Yishen Jianpi Xiezhuo Decoction on Renal Function and Pathological Changes in Rats with Gouty Nephropathy

Objective To investigate the effect of Yishen Jianpi Xiezhuo Decoction on renal function indexes and renal pathological changes in rats with gouty nephropathy. Methods 60 SD male rats were randomly divided into 6 groups: blank group, model group, positive control group(Febuxostat Tablets group), and Yishen Jianpi Xiezhuo Decoction of high, medium and low dose groups. The gouty nephropathy model rats were made by high yeast powder combined with adenine. When the gouty nephropathy model was established successfully, the rats were administrated by gavage according to the Febuxostat Tablets group (3.6 mg/kg/d), the high, medium and low dose groups (traditional Chinese medical extract 5.86 g/kg/d + Panax Notoginseng powder 0.54 g/kg/d, traditional Chinese medical extract 2.93 g/kg/d + Panax Notoginseng powder 0.27 g/kg/d, traditional Chinese medical extract 1.47 g/kg/d + Panax Notoginseng powder 0.14 g/kg/d) , respectively. After 21 d of continuous intervention, the serum uric acid (SUA), serum creatine(SCr) and blood urea nitrogen (BUN) were tested. The renal index were calculated and the pathological changes were observed. Results Compared with the blank group, the SUA, SCr and BUN increased significantly(P<0.05). Compared with the model group, the levels of SUA and BUN decreased in the high, middle and low dose groups of Yishen Jianpi Xiezhuo Decoction(P<0.05). The levels of SCr decreased in the high and middle dose groups of Yishen Jianpi Xiezhuo Decoction significantly(P<0.05). There was a downward trend in the level of SCr in the low dose group without statistically significant.The pathological changes of kidney in each group were improved in different degrees. Conclusion Yishen Jianpi Xiezhuo Decoction could reduce the levels of SUA, BUN and SCr, improve the renal pathological damage and protect the renal function of rats with gouty nephropathy.