| CTGF反义寡核苷酸治疗肺纤维化的实验研究 |
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| 引用本文： | 金哲,秦培顺,张志军.CTGF反义寡核苷酸治疗肺纤维化的实验研究[J].吉林医学,2006,27(1):6-7. |
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| 作者姓名： | 金哲 秦培顺 张志军 |
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| 作者单位： | 辽宁省电力中心医院 辽宁沈阳110015(金哲)，温州医学院附属第二医院 浙江温州325027(秦培顺)，吉林大学第一医院 吉林长春130021(张志军) |
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| 摘 要： | 目的:观察CGTF反义寡核苷酸对体外培养的人肺成纤维细胞的抗增殖作用。方法:将人肺成纤维细胞随机分为对照组、空载体转染组、CTGF反义寡核苷酸(CTGF-ASODN)组、CTGF正义寡核苷酸(CTGF-SODN)组。CTGF错义寡核苷酸(CT-GF-SCDN)组,转染后观察细胞的增殖程度以及CTGF mRNA的表达。结果:转染了CTGF反义寡核苷酸(CTGF-ASODN)组人成纤维细胞的增殖明显受到抑制,其CTGF mRNA的表达明显减少。结论:应用CGTF反义寡核苷酸可以起到对人肺成纤维细胞的抗增殖作用。
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| 关 键 词： | CTGF 反义寡核苷酸 转染 人肺成纤维细胞 增殖 |
| 文章编号： | 1004-0412（2006）01-06-02 |
| 收稿时间： | 2005-07-04 |
| 修稿时间： | 2005年7月4日 |
A study on pulmonary fibrosis cured by CTGF-ASODN |
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| JIN Zhe,QIN Pei-shun,ZHANG Zhi-jun.A study on pulmonary fibrosis cured by CTGF-ASODN[J].Jilin Medical Journal,2006,27(1):6-7. |
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| Authors: | JIN Zhe QIN Pei-shun ZHANG Zhi-jun |
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| Institution: | 1.Liaoning Electric Power Hospital,Liaoning Province,Shenyang 110015,China;2. The Second Affliated Hospital of Wenzhou Medical College ,Zhejiang Province ,Wenzhou 325057,China;3. The First Affliated Hospital of Jilin university Jilin Changchun 130021,China |
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| Abstract: | Objective To investigate the anti-proliferation effect on human lung fibroblast cultured in vitro cured by CTGF-ASODN.Method Human lung fibroblasts divided into control groups,empty carrier transfection groups,CTGF-ASODN groups,CTGF-SODN groups,CTGF-SCDN groups randomly.The extent of proliferation and the expression of CTGF mRNA were observed after transfection.Results The human lung fibroblasts transfected by CTGF-ASODN were inhibited significantly.The expression of CTGF mRNA was decreased obviously.Conclusion CTGF-ASODN can anti-proliferate to the human lung fibroblasts. |
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| Keywords: | CTGF |
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