下丘脑神经元钙激活钾通道的温度效应

目的研究新生SD乳鼠下丘脑神经元钙激活钾通道的温度敏感性.方法采用膜片钳技术内面向外式.结果随着温度的增加,通道的电导及开放概率显着增加.37℃、39℃时电导的Q₁₀为1.63和1.86,开放概率的Q₁₀为2.83和3.56,通道的开放、关闭时间都具有温度依赖性缩短.结论下丘脑钙激活钾通道具有高度的温度敏感性,对它的进一步研究可能有助于阐明体温调节的本质因素.

Effects of Temperature on Calcium-activated Potassium Channels(KCa) in Hypothalamic

ObjectiveTo study the effects of temperature on calcium-activated potassium channels(KCa)in hypothalamic neurons of new-born SD rats.MethodsThe data was recorded by adopting inside-out configuration of patch-clamp technique.ResultsAs the temperature increased,peak conductance and open probability(P₀)of the channel increased greatly.When the temperature was at 37℃ and 39℃,the Q₁₀ of the conductance and P₀ was 1.63、1.86 and 2.83、3.56.The time constants had temperature-dependent reductions.ConclusionThese results indicated the calcium2activated potassium channels in hypothalamic neurons had high temperature sensitivity,more researches on it may be helpful to elucidate the mechanism of temperature regulation of animals.