六味地黄汤含药血清对海马神经元突触活动的影响

目的：研究六味地黄汤益智作用的机理。方法：应用血清药理学和电生理方法，观察六味地黄汤含药血清对原代培养大鼠海马神经元自发突触活动的影响。结果：含药血清作用48h可明显增加海马神经元自发动作电流(spontaneous action cur-rent,sAC)和微小兴奋性突触后电流(miniature excitatory post aynaptic currenl,mEPSC)的产生频率，但并不影响mEPSC的幅度。结论：六味地黄汤可作用于突触前位点对海马神经元突触活动产生调节作用，该作用可能是其调节海马神经元的兴奋性进而发挥益智作用的机制之一。

Effect of the Serum from Liuwei Dihuang Decoction-treated Rats on Excitatory Synaptic Activity of Hippocampal Neurons

Aim To investigate the mechanism underlying the cognition|enhancing effect of Liuwei Dihuang decoction (LW).Method Whole|cell recording technique was used to observe the spontaneous synaptic activity of rat hippocampal neurons cultured with the serum from LW|treated rats,termed LW|containing serum(LWCS).Results After the hippocampal neurons were treated with LWCS for 48h,the frquency of spontaneous action current(sAC) and miniature excitatory post|synaptic current(mEPSC) were significantly increased,but the amplitude of mEPSC remained unchanged.Conclusion LWCS enhanced spontaneous synaptic activity of the hippocampal neurons possibly by affecting the pre|synaptic site of the neurons and this may contribute to the cognition|enhancing effect of LW.