甘草酸二铵磷脂复合物对小鼠慢性肝损伤的保护作用

目的：探讨甘草酸二铵磷脂复合物注射剂（DG-PC）对C57BL/6J小鼠慢性免疫性肝脏损害的保护作用。方法：复制C57BL/6J小鼠慢性免疫性肝脏损害模型,测定血清ALT、AST、蛋白和透明质酸（HA）,肝匀浆中超氧化物岐化酶（SOD）、谷胱甘肽（GSH）、I型胶原（C.I）,并对肝脏组织切片进行电镜观察。结果：DG-PC可以明显改善肝损小鼠AST水平、提高白蛋白（ALB）含量、升高白球比（A/G）、降低HA含量（P〈0.01）,提高肝损小鼠的SOD和GSH水平（P〈0.01）,治疗效果优于甘草酸二铵（DG）。小鼠肝组织透射电镜观察显示DG-PC可以保护肝细胞和细胞器,减轻纤维化程度。结论：DG-PC对于C57BL/6J小鼠慢性免疫性肝损伤均具有一定的保护作用,可能与抗氧化等活性有关。

Protective effects of diammonium glycyrrhizinate phospholipid complex on chronic liver injury

AIM: To approach the protective effects of diammonium glycyrrhizinate phospholipid complex injection (DG-PC) on chronic and immunological liver injury. METHODS: The chronic and immunological hepatic liver injury was established in C_(57)BL/6J mice, the levels of ALT, AST, TP,ALB and HA in serum were determined, the contents of SOD, GSH and collagen 1 (C.I) in liver homogenate were detected, and the liver tissue sections were observed by electron microscope. RESULTS: Compared with DG, DG-PC could significantly improve the AST level, increase the content of ALB, increase the A/G, decrease the content of HA(P < 0.01) , improve the activities of SOD and GSH(P < 0.01). The therapeutic efficacy of DG-PC was better than that of DG. As to microstructure, DG-PC could protect the hepatic cell and organelle, and relieve the fibrosis level. CONCLUSION: DG-PC has protective effects on chronic and immunological liver damage to C_(57) BL/6J mice, which maybe relate to the antioxidant effects.