慢性间歇低氧训练对大鼠心肌线粒体ATP酶的影响

目的: 研究慢性间歇低氧训练对大鼠心肌线粒体ATP酶含量及Na+、K+-ATP酶和Ca2+、Mg2+-ATP酶的影响。方法: 32只雄性Wistar大鼠按低氧训练方案分为慢性组11只、急性组12只、对照组9只。慢性组行慢性间歇低氧训练, 首先置于低压氧舱, 模拟海拔3 km, 每日持续4 h, 训练2周;再模拟海拔5 km, 每日持续4 h, 训练2周;最后模拟海拔8 km, 放置4 h。急性组立即置于模拟海拔8 km, 放置4 h。对照组则不行低氧训练。低氧期满后, 断头处死大鼠, 分离心肌线粒体, 测定ATP酶活性。结果: 慢性组ATP酶含量(9.04±4.71)μmol·mg-1·h-1, 均较急性组(4.96±1.17)μmol·mg-1·h-1和对照组(4.38±0.95)μmol·mg-1·h-1明显升高(P< 0.01)。慢性组Na+、K+-ATP酶活性(2.55±1.41)μmol·mg-1·h-1, 与急性组(2.66±1.07)μmol·mg-1·h-1和对照组(3.08±1.37)μmol·mg-1·h-1差异均无统计学意义(P>0.05)。慢性组Ca2+、Mg2+-ATP酶活性(1.17±0.34)μmol·mg-11·h-1与对照组(1.28±0.42)μmol·mg-1·h-1差异无统计学意义(P>0.05), 但较急性组(0.58±0.14)μmol·mg-1·h-1明显升高(P< 0.01)。结论: 慢性间歇低氧训练可增强Ca2+、Mg2+-ATP酶活性, 同时能够显著增加ATP酶含量, 有助于提高心肌运动功能, 使大鼠适应低氧环境。

Effect of chronic intermittent hypoxia training on rat myocardial mitochondria ATPase

Objective:To study the effects of chronic intermittent hypoxia training on rat myocardial ATP content Na+,K+-ATPase and Ca2+,Mg2-ATPase activities. Methods:Thirty-two male Wistar rats were divided into chronic group,acute group and control group according to the low oxygen training program,The rats in chronic group were subjected to chronic intermittent hypoxia training,daily training at 3 km simulated altitude for 4 h for 2 weeks,then daily training under 5 km simulated altitude for,4 h for the next 2 weeks, finally placed for 4 h under 8 km simulated altitude. The rats in acute group were immediately under 8 km simulated altitude for 4 h, and the rats in control group were no hypoxic training. After the expiry of the low oxygen, the rats were executed, myocardial mitochondria were separated and ATP enzyme activities were determined. Results:In chronic group,ATP enzyme content was(9. 04 ± 4.71) μmol·mg-1·h-1,which was higher than(4.96 ±1.17) μmol·mg-1·h-1 in acute group and(4.38 ±0.95) μmol·mg-1 ·h-1 in control group(P<0.01).In chronic group,Na+,K+ ATPase activity was(2.55 ±1.41) μmol·mg-1·h-1,and(2.66 ± 1. 07) μmol·mg-1·h-1 in acute group,(3. 08 ± 1. 37) μmol· mg-1 ·h-1 in control group,there had no statistical difference among the three groups(P>0.05).In chronic group,Ca2+,Mg2+ ATPase activity was(1.17±0.34) μmol·mg-1·h-1,and(1.28±0.42) μmol·mg-1·h-1 in control group,which had no statistical significance between the two groups(P>0. 05),but was higher than(0.58±0.14) μmol· mg-1·h-1 in acute group(P<0.01).Conclusions:Chronic intermittent hypoxia training can enhance Ca2+,Mg2+ ATPase activity,at the same time can significantly increase ATPase content,which can improve myocardial motion function, the rats can adapt the hypoxic environment.