糖尿病大鼠视网膜中IGF-1的动态表达及巴曲酶对其的影响

目的探讨胰岛素样生长因子（IGF-1）在糖尿病大鼠视网膜中的动态变化规律，初步证实巴曲酶对IGF-1的影响并探讨其机制，为临床上治疗糖尿病视网膜病变（DR）提供理论依据。方法将大鼠腹腔注射链脲佐菌素制作实验性糖尿病模型，再分别于成模后2个月和3个月时腹腔注射巴曲酶进行干预。通遏免疫组织化学和原位杂交双重检测IGF-1在视网膜中的表达。结果DM造模后2个月时视网膜中IGF-1的含量明显增加，且随时间变化有统计学差异，而巴曲酶治疗后IGF-1的表达明显增加。结论DR大鼠视网膜中IGF-1表达增加可能参与DR的发病机制。巴曲酶对DR有保护作用，其机制可能包括对IGF-1的调节。

The Dynamic Expression of IGF-1 and the Impact of Batroxobin on it in the Retina of Diabetic Rats

Objective To investigate the dy-namic regularity of IGF-1 in the retina of diabetic rats and to confirm the impact of Batroxobin on IGF-1 tentatively and the mechanism in order to furnish the theory foundation for the therapy of DR clinically. Mothoci Through the peritoneal in- jection of streptozocin to make the experimental diabetes models, we injected Batroxobin into ab- dominal cavity of rats differently 2 months and 3 months after the formation of DR models for in- tervention. Through the immunohistochemistry and hybridization in situ to detect the expression of IGF-1 in the retina of the DR rats. Result The content of IGF-1 in the retina of the DR rats de- creased obviously 2 months after the formation of DM models and it had time-varying statistics di- versity, however the expression of IGF-1 was markedly increased after the treatment of Batrox-obin. Conclusion The decreased ,expression of IGF-1 in the retina of DR rats might play an important role in the pathogenesis of DR. Treatment with Batroxobin is beneficial in the prevention of DR,possibly through the regulation of the expression level of IGF-1 in the retina of the DR rats.