内源性大麻素对体外培养的牛眼小梁细胞PGE2表达及细胞骨架的影响

目的:研究内源性大麻素N-花生四氢酸氨基乙醇(anan-damide,AEA)对体外培养的牛眼小梁细胞细胞骨架及PGE2表达的影响。方法:对牛眼小梁细胞进行原代及传代培养,并行鉴定。对传3代的牛眼小梁细胞分别施加AEA浓度分别为0,0.1,1.0,10μmol/L的无血清培养液,作用24h后提取细胞上清液,并将细胞置于倒置显微镜下摄像,计算机图像分析系统计算细胞面积。用ELISA法检测PGE2浓度的变化。结果:AEA可以产生明显的细胞舒张效应,呈浓度依赖性。而且在一定范围内可以成剂量依赖性的促进PGE2的表达。与对照组均有显著性差异(P<0.05)。结论:AEA可引起小梁细胞的舒张和促进PGE2的释放。

Effect of AEA on the expression of PGE2 and cytoskeleton in cultured bovine trabe-cular cells

·AIM:To study the effect of anandamide(AEA)on the expression of PGE2 and cytoskeleton in cultured bovine trabecular meshwork cells(BTM cells)..METHODS:BTM cells were primarily cultured and subcultured.The third generation BTM cells were incubated with different dosages of AEA(0,0.1,1.0,10μmol/L,final concentration,diluted by DMEM)for 24 hours.Then the supernatant was collected.The morphology of BTM cells was observed by inverted microscope and the area of cells was calculated by computer image analytic system.The concentration of PGE2 in the medium was measured by the ELISA method..RESULTS:Compared with the control group,AEA could obviously induce the relaxation of BTM cells in a concentration-dependent manner.Moreover,in a certain range AEA can increase the expression of PGE2 in a dose-dependent manner..CONCLUSION:AEA may cause the relaxation of TM cells and promote the release of PGE2..