核转录因子κB在门静脉高压大鼠肺血管中的表达及意义

目的:探讨核转录因子-κB(NF-κB)在门脉高压大鼠肺血管中的表达及意义。方法:建立门脉高压大鼠模型,将实验动物随机分为三组:实验模型组,银杏治疗组,对照组,动态观察其肺血管NF-κBp65的表达。结果:对照组平均光密度值0.2229±0.0008、实验组0.3007±0.0044、治疗组0.2866±0.0037。造模后第2周、3周、4周,实验组为:0.2739±0.0383、0.2982±0.0754、0.3202±0.0592;治疗组为:0.2625±0.0534、0.2890±0.0686、0.2918±0.0683。对三组结果进行两两非配对t检验,都具有显著性差异(P<0.01);相同时间段实验组与治疗组间亦具有显著性差异(P<0.01)。结论:门脉高压可促成肺血管组织NF-κBp65的高表达,并在门脉高压引起的血管病变中发挥重要作用;银杏注射液对NF-κBp65的高表达有抑制作用。

Expression of NF-κB in Pulmonary Vessels of Rats with Portal Hypertension and Its Implications

Objective: To investigate the expression of NF-κB in pulmonary vessels of rats with portal hypertension(PH) and its implications.Method: Rats were randomly allocated to three groups:①Experimental group;②Treatment group;③Control group. Detected the expression of NF-κB by immunohistochemistry.Results: There are significant difference between each two groups(P<0.01), even during the same period of time there are significant difference too(P<0.01).Conclusion: PH can cause over expression of NF-κB in pulmonary vessels of rats.NF-κB may play an important role in PH vasculopathy. Therapeutic treatment of Gingko injection can inhibite over-expression of NF-κB p65 induced by PH.