间质性肺疾病患者血清和支气管肺泡灌洗液中内皮素1活性的变化

目的评价内皮素对肺纤维化发生、发展作用的影响。方法利用同位素放射免疫直接测定法，检测１０例肺结节病和８例特发性肺纤维化（ＩＰＦ）患者外周血和支气管肺泡灌洗液（ＢＡＬＦ）中内皮素１（ＥＴ１）的活性，并与８名健康非吸烟者进行对照。结果肺结节病和ＩＰＦ患者血清和ＢＡＬＦ中的ＥＴ１活性分别为（６２±２９）ｎｇ／Ｌ，（１７０±２４）ｎｇ／Ｌ和（７７±７１）ｎｇ／Ｌ、（１０±３）ｎｇ／Ｌ，与正常对照组（２０±８）ｎｇ／Ｌ、（４０±０６）ｎｇ／Ｌ比较，差异有显著性（Ｐ＜００１）；血清中ＥＴ１活性与动脉血氧分压（ＰａＯ２）呈明显负相关（ｒ＝－０５３８，Ｐ＜００１）；结节病组和ＩＰＦ组ＢＡＬＦ中的ＥＴ１水平与ＢＡＬＦ中细胞总数呈正相关（ｒ＝０６４９，Ｐ＜００１），肺结节病患者、ＩＰＦ患者ＢＡＬＦ中ＥＴ１与淋巴细胞、中性粒细胞呈正相关（ｒ＝０７１２，０８１３，Ｐ均＜００１）。结论ＥＴ１在肺结节病和ＩＰＦ发病机制中起着重要作用，并可作为疾病活动性判定的一项重要参考指标。

Changes of the activities of ET1 in the serum and BALF of ILD patients and their clinical significance

Objective To evaluate the roles of the Endothelin1 (ET1) in pathogenesis of the patients with interstitial lung disease (ILD). Methods The ET1 activities in the serum and BALF from 10 patients with sarcoidosis, 7 with idiopathic pulmonary fibrosis (IPF) and 8 normal subjects nonsmokers were determined using the radioimmunoassay. Results In the activities of ET1 in the serum and BALF in the patients with sarcoiosis (6229 and 17024) ng/L and in the IPF (7771 and 103) ng/L. was higher than those in the normal control subjects (208 and 4006) ng/L. The activities of ET1 in serum was negative correlated with PaO2(r=-0538,P<001). The level of ET1 in the BALF of the patient was positive correlated with total cells in BALF (r=0649, P<001). The levels of ET1 in the BALF in the sarcoidosis was positive correlated with the percentage of lymphocyte (r=0712, P<001) but in patients with IPF was positive correlated with the percentage of neutrophil (r=0813, P<001). Conclusions ET1 might play an important role in pathogenic of the patients with sarcoidosis and IPF. The levels of ET1 can act as the markers of activity of disease.