| 测定环孢素全血浓度的2种方法对比及临床应用 |
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| 引用本文： | 张静,王羽,王璐璐,吴宝萍.测定环孢素全血浓度的2种方法对比及临床应用[J].中国新药与临床杂志,2011(5). |
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| 作者姓名： | 张静 王羽 王璐璐 吴宝萍 |
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| 作者单位： | 南京大学医学院附属鼓楼医院药剂科; |
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| 摘 要： | 目的对比荧光偏振免疫法(FPIA)和均相酶扩大免疫分析法(EMIT)2种测定方法监测环孢素(CsA)血药浓度的差异。方法收集98例次环孢素全血样品,同日同一患者的全血样品分别采用FPIA和EMIT法测定血药浓度。结果 EMIT和FPIA测定结果相关系数r为0.9795,EMIT法较FPIA法平均低(11±12)%(P<0.05,n=98),其中CsA血药谷值浓度(c_0)EMIT法较FPIA法平均低(14±9)%(P<0.05,n=69)。CsA血药峰值浓度(c_2)EMIT法较FPIA法平均低(2±5)%(P>0.05,n=29)。结论 2种不同方法测定全血中CsA血药浓度具有显著差异,EMIT法较FPIA法具有较高的特异性。
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| 关 键 词： | 环孢素A 荧光偏振免疫法 均相酶扩大免疫分析法 |
Comparison of two immunoassay methods for determination of cyclosporine in whole blood |
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| ZHANG Jing,WANG Yu,WANG Lu-lu,WU Bao-ping.Comparison of two immunoassay methods for determination of cyclosporine in whole blood[J].Chinese Journal of New Drugs and Clinical Remedies,2011(5). |
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| Authors: | ZHANG Jing WANG Yu WANG Lu-lu WU Bao-ping |
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| Institution: | ZHANG Jing,WANG Yu,WANG Lu-lu,WU Bao-ping (Department of Pharmacy,Drum Tower Hospital Affiliated to Medical College of Nanjing University,Nanjing JIANGSU 210008,China) |
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| Abstract: | AIM To compare two immunoassay methods for the determination of cyclosporine(CsA) in whole blood,a fluorescence polarization immunoassay(FPIA) and a enzyme multiplied immunoassay technique (EMIT).METHODS CsA in blood samples from 98 patients with livier,kidney and/or hematopoietic stem cell transplants were determined by EMIT and FPIA,respectively.RESULTS The correlation coefficient of EMIT and FPIA was 0.979 5.The determination results of EMIT was lower than FPIA,and the mean differences were as follows:mi... |
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| Keywords: | cyclosporine A fluorescence polarization immunoassay enzyme multiplied immunoassay technique |
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