丹芍化纤对肺纤维化大鼠肺内结缔组织生长因子表达的影响

目的：观察辛伐他汀对博来霉素引起的肺纤维化大鼠肺内结缔组织生长因子（CTGF）表达的影响。方法：选取SD大鼠30只,随机分为正常对照组（对照组）、肺纤维化模型组（模型组）和丹芍化纤胶囊治疗组（治疗组）。模型组和治疗组气管内注射博来霉素（5 mg/kg）诱导肺纤维化,对照组气管内注射等量生理盐水。次日起治疗组大鼠给予丹芍化纤混悬液（0.8 g/kg/d）灌胃,其余两组给予等量生理盐水灌胃。治疗第28 d处死各组大鼠,计算肺系数,HE染色观察肺组织病理变化,碱水解法检测肺组织羟脯氨酸含量,免疫组化ABC法观察肺组织CTGF表达的变化。结果：与对照组比较,模型组大鼠肺系数明显增加,肺组织胶原沉积明显,羟脯氨酸含量、CTGF表达增加。与模型组比较,治疗组大鼠肺系数明显降低,肺组织胶原沉积有所减轻,肺组织羟脯氨酸含量、CTGF蛋白表达减少。结论：丹芍化纤具有较好的抗大鼠肺纤维化作用,其作用机制部分是通过下调肺组织内CTGF的表达,从而延缓甚至抑制纤维化的进展。

Effect of danshaohuaxian on the expression of CTGF in the lung of rats with fibrosis pulmonary

Objective:To investigate the effect of Danshaohuaxian(DSH) on CTGF in rats with pulmonary fibrosis and to explore the Possible mechanism.Methods:Thirty Spague-dawley rats were randomly divided into the control group,model group and treatment group.The model group and the treatment group were induced pulmonary fibrosis with bleomycin(5 mg/kg)endotracheally,while the control group was given with normal saline instead.From the second day of injection,the rats in the treatment group were administered intragastricly with DSH(0.8 g/kg/d).while the other rats were given normal saline instead.All rats were sacrificed on the twenty-eighth day,The lung coefficient were observed,the rat lung histopathology was examined with HEstaining,lung hydroxyproline(HyP)content was evaluated,the expression of CTGF were examined by immunohistochemical staining.Results:Compared with the control group,the lung coefficient and the HyP content and expressions ofCTGF were significantly increased,the degree of pulmonary alveoli inflammation and pulmonary fibrosis were detected in the model group.After 4 weeks DSH treatment,the lung coefficient were significantly decreased,the degree of pulmonary alveoli inflammation and pulmonary fibrosis was significantly relieved,Hyp content and expression of CTGF were obviously decreased in the treatment group.Conclusion:DSH has a good action against experimental pulmonary fibrosis which may happen through up-regulation the ratio of CTGF to inhibit the progress of the fibrosis.