IMPs, VIMs and SPMs: the diversity of metallo-β-lactamases produced by carbapenem-resistant Pseudomonas aeruginosa in a Brazilian hospital |
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| Authors: | H S Sader A O Reis S Silbert A C Gales |
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| Institution: | Laboratorio Alerta e Laboratorio Especial de Microbiologia Clínica, Disciplina de Doen?as Infecciosas e Parasitárias, Universidade Federal de S?o Paulo, S?o Paulo, Brazil. helio-sader@jmilabs.com |
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| Abstract: | Pseudomonas aeruginosa isolates (n=183), collected from bacteraemic patients hospitalised in Sao Paulo Hospital (Brazil) during 2000-2001, were screened for susceptibility to antimicrobial agents. The polymyxins were the most active compounds (100% susceptibility), followed by amikacin and cefepime (59.0%), meropenem (57.4%), and imipenem and gentamicin (55.2%). Imipenem-resistant isolates were ribotyped and screened for production of metallo-beta-lactamases (MBLs) by PCR with primers for bla(IMP), bla(VIM) and bla(SPM). MBL production was detected in 36 isolates (19.7% of the entire collection; 43.9% of the imipenem-resistant isolates) and the MBLs included SPM-1-like (55.6%), VIM-2-like (30.6%) and IMP-1-like (8.3%) enzymes. |
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| Keywords: | Carbapenem resistance imipenem resistance metallo-β-lactamases polymyxin Pseudomonas aeruginosa |
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