通过抑制ERK1/2通路诱导肝癌细胞SMMC-7721凋亡的研究

目的应用细胞外信号调节激酶(ERK)1/2抑制剂U0126研究ERK1/2在肝癌细胞增殖、凋亡中的作用。方法以肝癌SMMC-7721细胞株为材料,分为空白对照组及不同浓度的U0126处理组。以MTT检测细胞增殖情况,流式细胞仪分析细胞周期及细胞凋亡情况。结果不同浓度的U0126处理后均可明显抑制肝癌SMMC-7721细胞的增殖(P<0.05,P<0.01),使处于G0/G1期的细胞明显增多(P<0.05)且呈剂量依赖性,并诱发细胞凋亡发生(P<0.05)。结论阻断ERK1/2通路有可能成为肝癌治疗的重要手段。

Effects of Inhibit Extracellular-Signal Regulated Kinase 1/2 Pathway on Apoptosis of Hepatoma Carcinoma Cell SMMC-7721

Objective To observe the effects of extracellular-signal regulated kinase(ERK) 1/2 inhibitor U0126 on hepatoma carcinoma cell proliferation and apoptosis.Methods Hepatoma SMMC-7721 cell strain was divided into blank control group and different concentrations of U0126 groups.The proliferation inhibition was measured by MTT assay.FCM was used to analyze the cell cycle distribution and apoptosis.Results U0126 obviously inhibited cell proliferation,induced cell apoptosis and G0/G1 phase cell cycle arrest.There were significant differences between control group and different concentrations of U0126 groups on cell proliferation and apoptosis(P<0.05,P<0.01).Conclusion Blocking ERK1/2 pathway may be an important treatment strategy for liver cancer.