抗氧化剂PDTC对肝癌细胞株Hep3B增殖的影响及机制

目的探讨抗氧化剂二硫代氨基甲酸毗咯烷（PDTC）在肝细胞性肝癌化学预防中的作用及机制。方法将不同浓度PDTC、阿霉素及PDTC联用阿霉素作用于Hep3B细胞。采用MTT法检测细胞存活率；流式细胞术（FCM）检测凋亡率；电泳迁移率变动分析（EMSA）检测NF—KB活性。结果PDTC作用后Hep3B存活率明显降低，呈浓度依赖性，P〈0．01。阿霉素联用PDTC的细胞生长抑制率明显高于单用阿霉素（P〈0．01）。PDTC为10μmol／L时的细胞凋亡率明显低于50μmol／L时（P〈0．01）。EMSA示PDTC作用后NF-κB表达降低，不同浓度PDTC作用两两相比P〈0．01。结论PDTC能抑制肝癌细胞株Hep3B的增殖、促进细胞凋亡、增强阿霉素的细胞毒作用；其作用机制主要是抑制NF-κB激活。PDTC可用于肝癌的化学预防，联用阿霉素可进一步提高疗效。

Effects of antioxidant PDTC on the proliferation of hepatocellular carcinoma Hep3B cells line and its mechanism

Objective To investigate the role and mechanism of the antioxidant pyrrolidine dithiocarbamate(PDTC) in the chemoprevention of the hepatocellular carcinoma(HCC).Methods The cytotoxity of PDTC,adriamycin,or PDTC combined with adriamycin to Hep3B cells line were detected by MTT.The apoptotic rate was analyzed by FCM.The NF-kappa B activity in Hep3B cells as well as Hep3B cells treated by PDTC were measured by EMSA.Results PDTC inhibited the proliferation of Hep3B cells significantly in a dose-dependent manner...