茶多酚影响同型半胱氨酸对内皮细胞的作用

目的:探讨茶多酚能否减弱同型半胱氨酸(HCY)对内皮细胞(EC)的损伤作用。方法:体外培养牛主动脉内皮细胞,随机分为5组,正常对照组,用含20%小牛血清DMEM培养液培养;0.25 mmol/L HCY组,培养液中加入HCY使其终浓度为0.25 mmol/L;1 mg/L茶多酚 +0.25 mmol/L HCY组;2 mg/L茶多酚 +0.25 mmol/L HCY组;4 mg/L茶多酚 +0.25 mmol/L HCY组。培养72h后,用流式细胞仪检测细胞周期,观察细胞生长情况,同时检测培养液中的一氧化氮(NO)、 一氧化氮合酶(NOS)、内皮素(ET)、谷胱甘肽过氧化酶(GSH-Px)、丙二醛(MDA)浓度或活性。结果:浓度为1、2、4、8 mg/L茶多酚组与HCY组相比,(1)细胞形态规则性增强,立体感增加,细胞内颗粒减少;(2)细胞周期检测提示茶多酚组细胞S期比例增多;(3)功能实验提示,茶多酚组EC释放的血管舒张因子NO浓度较HCY组高;而血管收缩因子ET低;(4)MDA含量和GS H-Px活性无明显改变。结论:茶多酚有抑制HCY对EC的损伤作用。

THE PROTECTIVE EFFECTS OF TEA POLYPHENOLS ON CULTURED BOVINE ENDOTHELIAL CELLS IMPAIRED BY HOMOCYSTEINE

Objective: To study the possible protective effects of tea polyphenols (TP) on the cultured bovine endothelial cells impaired by homocysteine . Methods: Bovine aortic endothelial cells were cultured and treated with a concentrated homocysteine and TP for 72 h . The cultured cells and the contents in the medium were studied by FCM , meanwhile nitric oxide (NO), endothelin(ET),GSH-Px and MDA contents were determined. Results:(1) TP inhibit the aortic endothelial cells proliferation induced by homocysteine ; (2) Vascular relaxing factor NO content in culture media in TP group was significantly higher than that of homocysteine group; (3) Vasoconstrictor ET was markedly lower than that of homocysteine group ; (4) There were no differences in the activity of GSH-Px and contents of MDA between TP group and homocysteine group. Conclusion: TP diminish the harmful effects of homocysteine on the aortic endothelial cells.