P-cadherin和HSP27蛋白在胃癌组织的表达及临床意义

目的:探讨P钙黏蛋白(P-cadherin)与热休克蛋白27(HSP27)在胃癌组织中的表达及其与临床病理因素之间的关系,进而探讨其作为预后指标的意义。方法:建立组织芯片,通过免疫组化SP法检测75例无术前放化疗史的胃癌手术标本和75例正常胃组织中P-cadherin、HSP27的表达情况,并对随访患者进行生存分析。结果 :在胃癌组织及正常胃组织中,Pcadherin、HSP27的表达率分别为40.0%、82.7%(P<0.001)和64.0%、30.7%(P<0.001)。P-cadherin低表达和HSP27高表达与临床分期、分化程度和淋巴结转移相关(P<0.05),HSP27高表达还与肿瘤大小相关(P<0.05)。Pearson相关分析显示,P-cadherin与HSP27在胃癌中的表达呈负相关(P<0.05)。单因素Kaplan-Meier回归分析显示P-cadherin和HSP27与预后相关(P<0.05)。Cox单因素回归分析显示P-cadherin、HSP27、临床分期、肿瘤大小、有无淋巴结转移与患者预后相关(P<0.05);Cox多因素回归模型分析显示HSP27、临床分期与患者预后相关(P<0.05)。结论:在胃癌中,P-cadherin低表达、HSP27高表达与胃癌临床病理特征和生物学行为密切相关。P-cadherin与HSP27的表达在胃癌组织中呈负相关。P-cadherin和HSP27可作为判断患者预后的候选指标。

Expression and clinical significance of P-cadherin and HSP27 proteins in gastric carcinoma

Objective:This study investigated the expression and significance of both P-cadherin and HSP27 proteins in gastric carcinoma tissues as well as the relationships among the clinical pathologic factors, and discussed the meaning of being prognostic indicators. Methods: High throughput technology of tissue microarray and immunohistochemical SP method was employed to investigate the expression of P-cadherin and HSP27 proteins in 75 gastric carcinoma specimens from patients without chemo-radiation therapy and 75 non-tumor gastric tissues. Survival analysis was carried out on the follow-up of patients. Results: In gastric cancer tissue and normal gastric tissue, the expression rates of P-cadherin, HSP27 were detected to be 40.0% and 82.7%, 64.0% and 30.7%, respectively (both P < 0.001). High expression of P-cadherin and low expression of HSP27 were related to clinical stage, differentiation degree and lymph node metastasis (P < 0.05), the high expression of HSP27 was also correlated with tumor size (P <0.05). According to the results of Pearson correlation analysis, the expression rates of P-cadherin and HSP27 in gastric cancer were negatively correlated to each other (P < 0.05). Kaplan-Meier analyses showed that P-cadherin and HSP27 were significantly associated with the prognosis (P < 0.05). The results of univariate analysis of Cox showed that P-cadherin, HSP27, clinical stage, tumor size and lymph node metastasis were significantly correlated with the prognosis (P < 0.05); The multivariate analysis of Cox model indicated that HSP27 and clinical stage were significantly correlated with the prognosis (P < 0.05). Conclusion: The low expression of P-cadherin in gastric carcinoma and high expression of HSP27, which was closely related with the clinical pathological features and biological behaviors of gastric cancer. Expression of P-cadherin and HSP27 were negatively correlated in gastric carcinoma. P-cadherin and HSP27 could be used as the candidate index to judge the prognosis of patients with gastric carcinoma.