肠胃清抗结肠癌侵袭转移机制初步探讨

目的:肠胃清抗结肠癌侵袭转移的分子机制研究。方法:以人结肠癌细胞株Lo Vo为研究对象,实验分为空白组,肠胃清低、中、高剂量组(0.98,1.97,3.93 g·L-1),奥沙利铂组(9.23 mg·L-1),联合组(肠胃清中剂量联合奥沙利铂组);药物作用于结肠癌Lo Vo细胞后,采用RT-q PCR法,Western blot法检测肠胃清对侵袭转移相关的E钙黏蛋白(E-cadherin)及Wint/β-链蛋白(β-catenin)信号通路因子β-catenin,(基质金属蛋白酶-7)MMP-7 mRNA及蛋白的影响。结果:与空白组比较,肠胃清低、中、高剂量组、奥沙利铂组及联合组均升高Lo Vo细胞E-cadherin mRNA的表达(P0.01),均能降低β-catenin及MMP-7 mRNA的表达(P0.05,P0.01),联合组作用更强;与空白组比较,肠胃清低、中、高剂量组、奥沙利铂组及联合组均升高Lo Vo细胞E-cadherin蛋白的表达(P0.05),均能降低β-catenin及MMP-7蛋白的表达(P0.05,P0.01),联合组作用更强。结论:肠胃清可以增加E-cadherin的表达量,增强细胞间的黏附能力,同时肠胃清可抑制Wint/βcatenin信号通路活性,降低β-catenin,MMP-7的表达,与奥沙利铂有协同作用。

Primary Research on Mechanism of Changweiqing in Treating Colon Cancer Invasion and Metastasis

Objective: To explore the molecular mechanisms of Changweiqing in treating colon cancer invasion and metastasis. Method: The experiment included the blank group, the low-, medium-, high-dose Changweiqing groups (0.98, 1.97, 3.93 g·L^-1), the oxaliplatin group(9.23 mg·L^-1), and the combination group (1.97 g·L^-1 Changweiqing plus 9.23 mg·L^-1 oxaliplatin). The colon cancer LoVo cells were used in this study. The mRNA and protein of E-cadherin, Wint/beta chain protein signaling pathway factor β-catenin and matrix metalloproteinase-7 (MMP-7) were detected by RT-qPCR and Western blot assay. Result: Compared to the blank group, the mRNA, protein expression of E-cadherin increased, the mRNA expression of β-catenin and MMP-7 decreased in LoVo cells at all doses of Changweiqing groups, oxaliplatin group and combined group (P<0.05, P<0.01). Meanwhile, the results were better in the combination group. Conclusion: Changweiqing has synergetic effect with oxaliplatin by increasing the expression of E-cadherin and enhancing the adhesion between cells, inhibiting Wint/β-catenin signaling pathway activity, decreasing the expression of β-catenin and MMP-7.