Cloning and characterisation of the adenosyl phosphosulphate kinase gene from Aspergillus nidulans |
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| Authors: | David L Clarke Roger W Newbert G Turner |
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| Institution: | (1) Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, South Yorkshire, S10 2UH, UK Fax: ++44-114-2728697 e-mail: G.Turner@Sheffield.ac.uk, GB;(2) Barton Laboratory, Department of Plant Pathology, Cornell University, Geneva, NY, 14456, USA, US |
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| Abstract: | The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing
a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal
region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality
of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.
Received: 17 April / 29 August 1997 |
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| Keywords: | APS kinase ATP sulphurylase Aspergillus nidulans Allosteric inhibition |
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