| 维A酸对体外培养的人牙周韧带细胞的生物学作用 |
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| 引用本文： | 王燕,汪说之,杨丕山,李纾. 维A酸对体外培养的人牙周韧带细胞的生物学作用[J]. 上海口腔医学, 2004, 13(4): 297-300 |
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| 作者姓名： | 王燕 汪说之 杨丕山 李纾 |
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| 作者单位： | 山东大学口腔医学院,山东,济南,250012;武汉大学口腔医学院,湖北,武汉,430079 |
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| 摘 要： | 目的:探讨维A酸对体外培养的人牙周韧带细胞的生物学作用。方法:体外培养人牙周韧带细胞。分别以不同浓度的维A酸作用于细胞,在倒置显微镜下观察不同浓度的维A酸对细胞形态与排列的影响;并通过MTT法、酶动力学方法及考马斯亮蓝法检测维A酸对细胞增殖活性、碱性磷酸酶活性及蛋白质合成的影响。结果:维A酸能增强牙周韧带细胞的碱性磷酸酶活性,10-6mol／L浓度的维A酸对细胞的作用效果最明显(P<0.01);而对细胞形态与排列、增殖能力及总蛋白合成无影响。结论:维A酸可促进牙周韧带细胞向成骨样细胞方向分化。
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| 关 键 词： | 细胞培养 牙周韧带细胞 维A酸 碱性磷酸酶 |
| 文章编号： | 1006-7248(2004)04-0297-04 |
| 修稿时间： | 2003-11-21 |
The biological effect of retinoic acid on cultured human periodontal ligament cells |
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| WANG Yan,WANG Shuo-zhi,YANG Pi-shan,LI Shu.. The biological effect of retinoic acid on cultured human periodontal ligament cells[J]. Shanghai journal of stomatology, 2004, 13(4): 297-300 |
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| Authors: | WANG Yan WANG Shuo-zhi YANG Pi-shan LI Shu. |
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| Affiliation: | School of Stomatology, Shandong University, Jinan 250012, Shandong Province, China. wangyan1965@sdu.edu.au |
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| Abstract: | PURPOSE: To evaluate the biological effect of retinoic acid on cultured human periodontal ligament cells. METHODS: Human periodontal ligament cells were cultured and challenged by retinoic acid with different concentrations. The cell morphology was observed under phase-contrast microscope; the cell proliferation activity was examined using MTT; the alkaline phosphatase activity was measured by enzyme kinetics method; the total amount of protein was assayed at different times. RESULTS: Retinoic acid enhanced alkaline phosphatase activity and had no effect on morphology, proliferation and total protein synthesis of periodontal ligament cells. CONCLUSION: Retinoic acid improves periodontal ligament cells to differentiate into osteoblast like cells. |
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| Keywords: | Superior anterior teeth Cell culture Periodontal ligament cells Retinoic acid Alkaline phosphatase |
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